Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2833058..2833689 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag BCG_RS13285 Protein ID WP_003413174.1
Coordinates 2833312..2833689 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag BCG_RS13280 Protein ID WP_003413167.1
Coordinates 2833058..2833315 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS13245 2828316..2828630 + 315 WP_009937839.1 hypothetical protein -
BCG_RS13250 2828926..2830137 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BCG_RS13255 2830264..2830923 + 660 WP_003900846.1 LppA family lipoprotein -
BCG_RS13260 2830920..2831579 + 660 WP_003900847.1 hypothetical protein -
BCG_RS13265 2831576..2832238 + 663 WP_003900848.1 hypothetical protein -
BCG_RS13270 2832235..2832513 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BCG_RS13275 2832606..2833019 + 414 WP_003413164.1 PIN domain nuclease -
BCG_RS13280 2833058..2833315 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
BCG_RS13285 2833312..2833689 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
BCG_RS13290 2833705..2834079 - 375 WP_003413177.1 hypothetical protein -
BCG_RS13295 2834179..2834574 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
BCG_RS13300 2834571..2834816 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
BCG_RS13305 2835227..2835646 - 420 WP_003413190.1 A24 family peptidase -
BCG_RS13310 2835658..2836485 - 828 WP_010950736.1 shikimate dehydrogenase -
BCG_RS13315 2836463..2837716 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BCG_RS13320 2837709..2838221 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T21887 WP_003413174.1 NC_008769:2833312-2833689 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T21887 NC_008769:2833312-2833689 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT21887 WP_003413167.1 NC_008769:2833058-2833315 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT21887 NC_008769:2833058-2833315 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References