Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 94405..94930 Replicon plasmid p2
Accession NZ_CP084544
Organism Klebsiella michiganensis strain YL9

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag LD277_RS30420 Protein ID WP_008322213.1
Coordinates 94625..94930 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag LD277_RS30415 Protein ID WP_032934863.1
Coordinates 94405..94623 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD277_RS30400 (LD277_30440) 90319..91016 + 698 WP_223216367.1 IS1 family transposase -
LD277_RS30405 (LD277_30445) 90951..92633 - 1683 WP_238979847.1 diguanylate cyclase -
LD277_RS30410 (LD277_30450) 92846..93543 + 698 Protein_109 IS1-like element IS1B family transposase -
LD277_RS30415 (LD277_30455) 94405..94623 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LD277_RS30420 (LD277_30460) 94625..94930 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
LD277_RS30425 (LD277_30465) 94932..95246 + 315 WP_008322211.1 hypothetical protein -
LD277_RS30430 (LD277_30470) 95236..96027 + 792 WP_008322208.1 site-specific integrase -
LD277_RS30435 (LD277_30475) 96226..97566 - 1341 Protein_114 GntP family transporter -
LD277_RS30440 (LD277_30480) 97710..98456 - 747 Protein_115 IS5-like element IS5 family transposase -
LD277_RS30450 (LD277_30490) 99677..99793 - 117 Protein_117 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / rmtC / sul1 htpB 1..103463 103463
- inside IScluster/Tn - - 85074..99829 14755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T218867 WP_008322213.1 NZ_CP084544:94625-94930 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T218867 NZ_CP116113:102793-102942 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT218867 WP_032934863.1 NZ_CP084544:94405-94623 [Klebsiella michiganensis]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT218867 NZ_CP116113:c102748-102689 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References