Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5392225..5393029 Replicon chromosome
Accession NZ_CP084542
Organism Klebsiella michiganensis strain YL9

Toxin (Protein)


Gene name yeeV Uniprot ID A0A486SQP3
Locus tag LD277_RS25710 Protein ID WP_040217122.1
Coordinates 5392225..5392614 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7H4M120
Locus tag LD277_RS25715 Protein ID WP_014837280.1
Coordinates 5392670..5393029 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD277_RS25680 (LD277_25705) 5387267..5388061 + 795 WP_042933970.1 DNA/RNA non-specific endonuclease -
LD277_RS25685 (LD277_25710) 5388351..5388674 + 324 WP_014837285.1 endoribonuclease SymE -
LD277_RS25690 (LD277_25715) 5388833..5389267 + 435 WP_004098173.1 VOC family protein -
LD277_RS25695 (LD277_25720) 5389433..5390176 + 744 WP_014837284.1 hypothetical protein -
LD277_RS25700 (LD277_25725) 5390354..5391151 - 798 WP_014837283.1 helix-turn-helix transcriptional regulator -
LD277_RS25705 (LD277_25730) 5391293..5392138 - 846 WP_014837282.1 DUF4942 domain-containing protein -
LD277_RS25710 (LD277_25735) 5392225..5392614 - 390 WP_040217122.1 TA system toxin CbtA family protein Toxin
LD277_RS25715 (LD277_25740) 5392670..5393029 - 360 WP_014837280.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LD277_RS25720 (LD277_25745) 5393054..5393275 - 222 WP_014837279.1 DUF987 domain-containing protein -
LD277_RS25725 (LD277_25750) 5393289..5393768 - 480 WP_014837278.1 DNA repair protein RadC -
LD277_RS25730 (LD277_25755) 5393780..5394223 - 444 WP_014837277.1 antirestriction protein -
LD277_RS25735 (LD277_25760) 5394303..5394533 - 231 WP_014837276.1 DUF905 domain-containing protein -
LD277_RS25740 (LD277_25765) 5394557..5395381 - 825 WP_040217125.1 DUF932 domain-containing protein -
LD277_RS25745 (LD277_25770) 5395921..5396553 + 633 WP_071886307.1 inovirus Gp2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5388833..5417430 28597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(3-111)

Antitoxin

Antitoxin CbeA

(16-114)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14477.57 Da        Isoelectric Point: 8.9843

>T218865 WP_040217122.1 NZ_CP084542:c5392614-5392225 [Klebsiella michiganensis]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDELVIQQHIEAGISLADALNFIVEKYELVRTDRPGF
SIREQSPFITSIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Download         Length: 390 bp

>T218865 NZ_CP116112:72858-73010 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13416.24 Da        Isoelectric Point: 7.0268

>AT218865 WP_014837280.1 NZ_CP084542:c5393029-5392670 [Klebsiella michiganensis]
MSKKTTIATHDISESWWGLRRSVSACFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQSFPVLMKQLELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVTTE

Download         Length: 360 bp

>AT218865 NZ_CP116112:c72808-72746 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486SQP3

Antitoxin

Source ID Structure
AlphaFold DB A0A7H4M120

References