Detailed information of TA system TA218864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5336021..5336597
• Replicon: chromosome
• Accession: NZ_CP084542
• Organism: Klebsiella michiganensis strain YL9

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1D8JNH2
• Locus tag: LD277_RS25450
• Protein ID: WP_042933975.1
• Coordinates: 5336310..5336597 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: LD277_RS25445
• Protein ID: WP_014837314.1
• Coordinates: 5336021..5336323 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LD277_RS25425 (LD277_25450)	5331523..5332743	+	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
LD277_RS25430 (LD277_25455)	5332861..5333196	+	336	WP_014837316.1	endoribonuclease SymE	-
LD277_RS25435 (LD277_25460)	5333649..5334560	+	912	WP_014837315.1	acetamidase/formamidase family protein	-
LD277_RS25440 (LD277_25465)	5334557..5335900	+	1344	WP_004128506.1	APC family permease	-
LD277_RS25445 (LD277_25470)	5336021..5336323	-	303	WP_014837314.1	BrnA antitoxin family protein	Antitoxin
LD277_RS25450 (LD277_25475)	5336310..5336597	-	288	WP_042933975.1	BrnT family toxin	Toxin
LD277_RS25455 (LD277_25480)	5336856..5337299	-	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
LD277_RS25460 (LD277_25485)	5337293..5338201	-	909	WP_014837311.1	LysR family transcriptional regulator	-
LD277_RS25465 (LD277_25490)	5338289..5339071	+	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
LD277_RS25470 (LD277_25495)	5339219..5339803	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
LD277_RS25475 (LD277_25500)	5339949..5340749	+	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
LD277_RS25480 (LD277_25505)	5340746..5341264	+	519	WP_014837307.1	FidL-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IS/Tn	fosA	-	5331523..5337299	5776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T218864 WP_042933975.1 NZ_CP084542:c5336597-5336310 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T218864 NZ_CP116111:88117-88515 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.14 Da
• Isoelectric Point: 9.7962

>AT218864 WP_014837314.1 NZ_CP084542:c5336323-5336021 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSGIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT218864 NZ_CP116111:87890-88117 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A1D8JNH2