218862

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4640829..4641448 Replicon chromosome
Accession NZ_CP084542
Organism Klebsiella michiganensis strain YL9

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag LD277_RS22220 Protein ID WP_004099646.1
Coordinates 4641230..4641448 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag LD277_RS22215 Protein ID WP_004129911.1
Coordinates 4640829..4641203 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD277_RS22205 (LD277_22230) 4635987..4637180 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LD277_RS22210 (LD277_22235) 4637203..4640349 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
LD277_RS22215 (LD277_22240) 4640829..4641203 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
LD277_RS22220 (LD277_22245) 4641230..4641448 + 219 WP_004099646.1 HHA domain-containing protein Toxin
LD277_RS22225 (LD277_22250) 4641611..4642177 + 567 WP_014837607.1 maltose O-acetyltransferase -
LD277_RS22230 (LD277_22255) 4642149..4642283 - 135 WP_223226764.1 hypothetical protein -
LD277_RS22235 (LD277_22260) 4642304..4642774 + 471 WP_014228205.1 YlaC family protein -
LD277_RS22240 (LD277_22265) 4642749..4644203 - 1455 WP_014837606.1 PLP-dependent aminotransferase family protein -
LD277_RS22245 (LD277_22270) 4644305..4645003 + 699 WP_014228203.1 GNAT family protein -
LD277_RS22250 (LD277_22275) 4645000..4645140 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LD277_RS22255 (LD277_22280) 4645140..4645403 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T218862 WP_004099646.1 NZ_CP084542:4641230-4641448 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T218862 NZ_CP116110:c4196232-4196059 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT218862 WP_004129911.1 NZ_CP084542:4640829-4641203 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT218862 NZ_CP116110:c4196556-4196260 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References