Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 874656..875313 Replicon chromosome
Accession NZ_CP084542
Organism Klebsiella michiganensis strain YL9

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag LD277_RS04240 Protein ID WP_004854060.1
Coordinates 874903..875313 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag LD277_RS04235 Protein ID WP_004124953.1
Coordinates 874656..874922 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LD277_RS04220 (LD277_04225) 869890..870315 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
LD277_RS04225 (LD277_04230) 870436..873234 - 2799 WP_014839506.1 transcriptional regulator DagR -
LD277_RS04230 (LD277_04235) 873428..874411 - 984 WP_014226793.1 tRNA-modifying protein YgfZ -
LD277_RS04235 (LD277_04240) 874656..874922 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
LD277_RS04240 (LD277_04245) 874903..875313 + 411 WP_004854060.1 protein YgfX Toxin
LD277_RS04245 (LD277_04250) 875322..875843 - 522 WP_014226792.1 flavodoxin FldB -
LD277_RS04250 (LD277_04255) 875965..876861 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
LD277_RS04255 (LD277_04260) 876884..877597 + 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LD277_RS04260 (LD277_04265) 877603..879336 + 1734 WP_014226791.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T218853 WP_004854060.1 NZ_CP084542:874903-875313 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T218853 NZ_CP116110:1978231-1978334 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT218853 WP_004124953.1 NZ_CP084542:874656-874922 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT218853 NZ_CP116110:c1978372-1978101 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References