Detailed information of TA system TA021885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2818063..2818703
• Replicon: chromosome
• Accession: NC_008769
• Organism: Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: BCG_RS13185
• Protein ID: WP_003412970.1
• Coordinates: 2818063..2818482 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: BCG_RS13190
• Protein ID: WP_003412975.1
• Coordinates: 2818479..2818703 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCG_RS13160	2813648..2814370	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
BCG_RS13165	2814887..2815114	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
BCG_RS13170	2815111..2815512	+	402	WP_003412963.1	PIN domain-containing protein	-
BCG_RS13175	2815547..2816467	-	921	WP_011799273.1	restriction endonuclease	-
BCG_RS22280	2816808..2817053	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BCG_RS13180	2817112..2818062	+	951	WP_003911916.1	Lsr2 family protein	-
BCG_RS13185	2818063..2818482	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCG_RS13190	2818479..2818703	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
BCG_RS13195	2818734..2821577	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
BCG_RS13200	2821649..2822050	-	402	WP_003412981.1	hypothetical protein	-
BCG_RS13205	2822050..2822520	-	471	WP_003412985.1	transcription antitermination factor NusB	-
BCG_RS13210	2822523..2823086	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T21885 WP_003412970.1 NC_008769:c2818482-2818063 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T21885 NC_008769:c2818482-2818063 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT21885 WP_003412975.1 NC_008769:c2818703-2818479 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT21885 NC_008769:c2818703-2818479 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09