Detailed information of TA system TA218848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 307505..308144
• Replicon: chromosome
• Accession: NZ_CP084539
• Organism: Peribacillus frigoritolerans strain Ant232

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LDO17_RS01540
• Protein ID: WP_034306610.1
• Coordinates: 307794..308144 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LDO17_RS01535
• Protein ID: WP_034306380.1
• Coordinates: 307505..307786 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO17_RS01515	303643..304233	-	591	WP_048682037.1	rhomboid family intramembrane serine protease	-
LDO17_RS01520	304342..304692	+	351	WP_048682039.1	holo-ACP synthase	-
LDO17_RS01525	304935..305939	+	1005	WP_048682041.1	outer membrane lipoprotein carrier protein LolA	-
LDO17_RS01530	306157..307344	+	1188	WP_048682044.1	alanine racemase	-
LDO17_RS01535	307505..307786	+	282	WP_034306380.1	antitoxin EndoAI	Antitoxin
LDO17_RS01540	307794..308144	+	351	WP_034306610.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LDO17_RS01545	308558..309385	+	828	WP_048682050.1	RsbT co-antagonist protein RsbRA	-
LDO17_RS01550	309388..309744	+	357	WP_048682053.1	STAS domain-containing protein	-
LDO17_RS01555	309748..310149	+	402	WP_048682056.1	anti-sigma regulatory factor	-
LDO17_RS01560	310159..311169	+	1011	WP_048682059.1	PP2C family protein-serine/threonine phosphatase	-
LDO17_RS01565	311229..311561	+	333	WP_034306369.1	anti-sigma factor antagonist	-
LDO17_RS01570	311558..312031	+	474	WP_034306366.1	anti-sigma B factor RsbW	-
LDO17_RS01575	312009..312797	+	789	WP_034306363.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12955.06 Da
• Isoelectric Point: 7.2029

>T218848 WP_034306610.1 NZ_CP084539:307794-308144 [Peribacillus frigoritolerans]
IVVKRGDVYFADLSPVVGSEQGGTRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEPMMQKVNEALQISLGLIEF

Nucleotide

• Length: 351 bp

>T218848 NZ_CP116110:821254-821661 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10713.13 Da
• Isoelectric Point: 5.9054

>AT218848 WP_034306380.1 NZ_CP084539:307505-307786 [Peribacillus frigoritolerans]
VSESSATREILIRLPQNFLTELDGYASEENVNRSEFIYRATKMYLRERKKKEFRESMKRGYIEMAAINLTIASEAFQAEF
EAGHCVERLVSGG

Nucleotide

• Length: 282 bp

>AT218848 NZ_CP116110:821007-821273 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA