Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-RelB |
| Location | 4435128..4435654 | Replicon | chromosome |
| Accession | NZ_CP084529 | ||
| Organism | Escherichia coli strain Origami2-DE3 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | S1EXL1 |
| Locus tag | LFW28_RS21440 | Protein ID | WP_000323025.1 |
| Coordinates | 4435367..4435654 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | S1F6D3 |
| Locus tag | LFW28_RS21435 | Protein ID | WP_000534858.1 |
| Coordinates | 4435128..4435367 (+) | Length | 80 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LFW28_RS21380 (4430155) | 4430155..4430922 | - | 768 | Protein_4199 | exonuclease | - |
| LFW28_RS21385 (4431015) | 4431015..4431206 | - | 192 | WP_001083297.1 | lysis protein YdfD | - |
| LFW28_RS21390 (4431203) | 4431203..4431391 | - | 189 | WP_000854559.1 | cell division inhibition protein DicB | - |
| LFW28_RS21395 (4431959) | 4431959..4432177 | - | 219 | WP_001171942.1 | protein YdfC | - |
| LFW28_RS23145 (4432207) | 4432207..4432335 | - | 129 | WP_000344964.1 | protein YdfB | - |
| LFW28_RS21400 (4432337) | 4432337..4432492 | - | 156 | WP_000379575.1 | DUF1391 family protein | - |
| LFW28_RS21405 (4432659) | 4432659..4433066 | - | 408 | WP_000448564.1 | DNA-binding transcriptional dual regulator DicA | - |
| LFW28_RS21410 (4433150) | 4433150..4433380 | + | 231 | WP_000920568.1 | dicB transcriptional regulator DicC | - |
| LFW28_RS21415 (4433364) | 4433364..4433642 | + | 279 | Protein_4207 | hypothetical protein | - |
| LFW28_RS21420 (4433677) | 4433677..4433826 | + | 150 | WP_011443592.1 | protein YdfW | - |
| LFW28_RS21425 (4434263) | 4434263..4434595 | - | 333 | WP_001301033.1 | FlxA-like family protein | - |
| LFW28_RS21430 (4434798) | 4434798..4435103 | - | 306 | WP_001326990.1 | protein YdfV | - |
| LFW28_RS21435 (4435128) | 4435128..4435367 | + | 240 | WP_000534858.1 | type II toxin-antitoxin system antitoxin RelB | Antitoxin |
| LFW28_RS21440 (4435367) | 4435367..4435654 | + | 288 | WP_000323025.1 | type II toxin-antitoxin system mRNA interferase RelE | Toxin |
| LFW28_RS21445 (4435726) | 4435726..4435881 | + | 156 | WP_000813254.1 | type I toxin-antitoxin system toxin HokD | - |
| LFW28_RS21450 (4436098) | 4436098..4436349 | + | 252 | WP_000980994.1 | protein Rem | - |
| LFW28_RS21455 (4436416) | 4436416..4436694 | + | 279 | WP_012304870.1 | hypothetical protein | - |
| LFW28_RS21460 (4436696) | 4436696..4437745 | + | 1050 | WP_001265198.1 | DUF968 domain-containing protein | - |
| LFW28_RS21465 (4437759) | 4437759..4438511 | + | 753 | WP_001047135.1 | antitermination protein | - |
| LFW28_RS21470 (4438789) | 4438789..4438878 | - | 90 | WP_120795389.1 | hypothetical protein | - |
| LFW28_RS21475 (4438933) | 4438933..4439145 | - | 213 | WP_000087756.1 | cold shock-like protein CspF | - |
| LFW28_RS21480 (4439446) | 4439446..4439661 | + | 216 | WP_000066484.1 | cold shock-like protein CspB | - |
| LFW28_RS21485 (4440025) | 4440025..4440195 | - | 171 | WP_211180497.1 | putative zinc-binding protein YnfU | - |
| LFW28_RS21490 (4440415) | 4440415..4440630 | + | 216 | WP_000839590.1 | phage lysis protein EssD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11225.22 Da Isoelectric Point: 10.1967
>T218816 WP_000323025.1 NZ_CP084529:4435367-4435654 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
Download Length: 288 bp
>T218816 NZ_CP116104:c91859-91554 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 80 a.a. Molecular weight: 9071.48 Da Isoelectric Point: 4.5230
>AT218816 WP_000534858.1 NZ_CP084529:4435128-4435367 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL
Download Length: 240 bp
>AT218816 NZ_CP116104:c92079-91861 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|