Detailed information of TA system TA218809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3939441..3940066
• Replicon: chromosome
• Accession: NZ_CP084529
• Organism: Escherichia coli strain Origami2-DE3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LFW28_RS18905
• Protein ID: WP_000911324.1
• Coordinates: 3939668..3940066 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: LFW28_RS18900
• Protein ID: WP_000450532.1
• Coordinates: 3939441..3939668 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFW28_RS18900 (3939441)	3939441..3939668	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LFW28_RS18905 (3939668)	3939668..3940066	+	399	WP_000911324.1	type II toxin-antitoxin system VapC family toxin	Toxin
LFW28_RS18910 (3940075)	3940075..3942228	-	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
LFW28_RS18915 (3942481)	3942481..3943212	-	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
LFW28_RS18920 (3943237)	3943237..3943758	-	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	fimH / fimD / allD / allC / allB / allR / allA / allS / rhs/PAAR	3895626..4066367	170741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T218809 WP_000911324.1 NZ_CP084529:3939668-3940066 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T218809 NZ_CP116103:c2840220-2839969 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT218809 WP_000450532.1 NZ_CP084529:3939441-3939668 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT218809 NZ_CP116103:c2839972-2839718 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5