Detailed information of TA system TA218803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2668925..2670362
• Replicon: chromosome
• Accession: NZ_CP084529
• Organism: Escherichia coli strain Origami2-DE3

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: LFW28_RS12760
• Protein ID: WP_000155570.1
• Coordinates: 2668925..2669998 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: LFW28_RS12765
• Protein ID: WP_000461704.1
• Coordinates: 2669991..2670362 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFW28_RS12745 (2664358)	2664358..2666547	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
LFW28_RS12750 (2666544)	2666544..2668160	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
LFW28_RS12755 (2668520)	2668520..2668783	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
LFW28_RS12760 (2668925)	2668925..2669998	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
LFW28_RS12765 (2669991)	2669991..2670362	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
LFW28_RS12770 (2670717)	2670717..2671580	+	864	WP_000787597.1	GTPase family protein	-
LFW28_RS12775 (2671672)	2671672..2672493	+	822	WP_000197391.1	DUF932 domain-containing protein	-
LFW28_RS12780 (2672710)	2672710..2673411	+	702	WP_000189409.1	WYL domain-containing protein	-
LFW28_RS12785 (2673452)	2673452..2673688	+	237	WP_001144029.1	protein YpjK	-
LFW28_RS12790 (2673688)	2673688..2674131	+	444	WP_000824222.1	lipoprotein YfjS	-
LFW28_RS12795 (2674155)	2674155..2674622	+	468	WP_000702495.1	protein YfjT	-
LFW28_RS12800 (2675009)	2675009..2675161	-	153	Protein_2514	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T218803 WP_000155570.1 NZ_CP084529:2668925-2669998 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T218803 NZ_CP116103:c1203320-1203102 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT218803 WP_000461704.1 NZ_CP084529:2669991-2670362 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT218803 NZ_CP116103:c1203720-1203346 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• AlphaFold DB: A0A373F4G4