Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yjhX-yjhQ/YjhX-GNAT
Location 2224683..2225497 Replicon chromosome
Accession NZ_CP084529
Organism Escherichia coli strain Origami2-DE3

Toxin (Protein)


Gene name yjhX Uniprot ID S1PA82
Locus tag LFW28_RS10590 Protein ID WP_001054376.1
Coordinates 2224683..2224940 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name yjhQ Uniprot ID U9Z4B8
Locus tag LFW28_RS10595 Protein ID WP_001309181.1
Coordinates 2224952..2225497 (+) Length 182 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFW28_RS10565 (2219971) 2219971..2221077 + 1107 WP_001309184.1 N-acetylneuraminate epimerase -
LFW28_RS10570 (2221142) 2221142..2222122 + 981 WP_000991438.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
LFW28_RS10575 (2222232) 2222232..2222437 + 206 Protein_2081 HNH endonuclease -
LFW28_RS10580 (2222705) 2222705..2223945 - 1241 Protein_2082 helicase YjhR -
LFW28_RS10585 (2224061) 2224061..2224192 + 132 WP_001309182.1 hypothetical protein -
LFW28_RS10590 (2224683) 2224683..2224940 + 258 WP_001054376.1 YjhX family toxin Toxin
LFW28_RS10595 (2224952) 2224952..2225497 + 546 WP_001309181.1 N-acetyltransferase Antitoxin
LFW28_RS10600 (2225553) 2225553..2226299 + 747 WP_000354251.1 class I SAM-dependent methyltransferase -
LFW28_RS10605 (2226468) 2226468..2226686 + 219 Protein_2087 hypothetical protein -
LFW28_RS10610 (2226724) 2226724..2226840 + 117 Protein_2088 VOC family protein -
LFW28_RS10615 (2227085) 2227085..2228206 + 1122 WP_000010829.1 M42 family metallopeptidase -
LFW28_RS10620 (2228203) 2228203..2228481 + 279 WP_000722973.1 PTS sugar transporter subunit IIB SgcB -
LFW28_RS10625 (2228493) 2228493..2229806 + 1314 WP_000460843.1 PTS sugar transporter subunit IIC SgcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimI / fimA / fimE / fimB 2214469..2233721 19252


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Putative toxin of bacterial toxin-antitoxin pair

(1-85)

Antitoxin

GNAT domain

(18-121)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 9734.29 Da        Isoelectric Point: 11.0090

>T218800 WP_001054376.1 NZ_CP084529:2224683-2224940 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Download         Length: 258 bp

>T218800 NZ_CP116103:c692165-692013 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 182 a.a.        Molecular weight: 19956.90 Da        Isoelectric Point: 6.3277

>AT218800 WP_001309181.1 NZ_CP084529:2224952-2225497 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Download         Length: 546 bp

>AT218800 NZ_CP116103:692214-692271 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PA82

Antitoxin

Source ID Structure
AlphaFold DB U9Z4B8

References