Detailed information of TA system TA218797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1749121..1749739
• Replicon: chromosome
• Accession: NZ_CP084529
• Organism: Escherichia coli strain Origami2-DE3

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: LFW28_RS08455
• Protein ID: WP_001290581.1
• Coordinates: 1749121..1749339 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: LFW28_RS08460
• Protein ID: WP_000344800.1
• Coordinates: 1749365..1749739 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFW28_RS08420	1744410..1744982	+	573	WP_000779842.1	YbaY family lipoprotein	-
LFW28_RS08425	1745013..1745324	-	312	WP_000409911.1	MGMT family protein	-
LFW28_RS08435	1745703..1746056	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
LFW28_RS08440	1746098..1747648	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
LFW28_RS08445	1747812..1748282	-	471	WP_000136192.1	YlaC family protein	-
LFW28_RS08450	1748398..1748949	-	552	WP_000102564.1	maltose O-acetyltransferase	-
LFW28_RS08455	1749121..1749339	-	219	WP_001290581.1	HHA domain-containing protein	Toxin
LFW28_RS08460	1749365..1749739	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
LFW28_RS08465	1750285..1753434	-	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
LFW28_RS08470	1753457..1754650	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T218797 WP_001290581.1 NZ_CP084529:c1749339-1749121 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T218797 NZ_CP116103:c315790-315416 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT218797 WP_000344800.1 NZ_CP084529:c1749739-1749365 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT218797 NZ_CP116103:c316248-315880 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5