Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1618609..1619263 Replicon chromosome
Accession NZ_CP084529
Organism Escherichia coli strain Origami2-DE3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LFW28_RS07840 Protein ID WP_000244777.1
Coordinates 1618856..1619263 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LFW28_RS07835 Protein ID WP_000354046.1
Coordinates 1618609..1618875 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFW28_RS07810 (1613778) 1613778..1614521 + 744 WP_000951964.1 SDR family oxidoreductase -
LFW28_RS07815 (1614578) 1614578..1616011 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
LFW28_RS07820 (1616056) 1616056..1616367 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LFW28_RS07825 (1616531) 1616531..1617190 + 660 WP_000250274.1 hemolysin III family protein -
LFW28_RS07830 (1617386) 1617386..1618366 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LFW28_RS07835 (1618609) 1618609..1618875 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LFW28_RS07840 (1618856) 1618856..1619263 + 408 WP_000244777.1 protein YgfX Toxin
LFW28_RS07845 (1619303) 1619303..1619824 - 522 WP_001055874.1 flavodoxin FldB -
LFW28_RS07850 (1619936) 1619936..1620832 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LFW28_RS07855 (1620857) 1620857..1621567 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LFW28_RS07860 (1621573) 1621573..1623306 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T218796 WP_000244777.1 NZ_CP084529:1618856-1619263 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T218796 NZ_CP116103:304280-304453 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT218796 WP_000354046.1 NZ_CP084529:1618609-1618875 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT218796 NZ_CP116103:303956-304252 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References