Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2212960..2213546 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag BCG_RS10335 Protein ID WP_003410010.1
Coordinates 2212960..2213304 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag BCG_RS10340 Protein ID WP_003410014.1
Coordinates 2213298..2213546 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS10305 2209861..2210166 + 306 Protein_2014 ABC transporter permease -
BCG_RS10310 2210350..2210874 + 525 WP_011799234.1 methyltransferase -
BCG_RS10315 2211394..2211954 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
BCG_RS10320 2211951..2212292 - 342 WP_003410003.1 DUF2384 domain-containing protein -
BCG_RS10325 2212378..2212635 + 258 WP_003410006.1 hypothetical protein -
BCG_RS10330 2212536..2212871 - 336 WP_003410009.1 dehydrogenase -
BCG_RS10335 2212960..2213304 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
BCG_RS10340 2213298..2213546 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
BCG_RS10345 2213646..2215961 - 2316 WP_003899120.1 cation transporter ATPase CptG -
BCG_RS10350 2215958..2216230 - 273 WP_003410017.1 DUF1490 family protein -
BCG_RS10355 2216283..2216639 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
BCG_RS10360 2216796..2217563 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T21878 WP_003410010.1 NC_008769:c2213304-2212960 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T21878 NC_008769:c2213304-2212960 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT21878 WP_003410014.1 NC_008769:c2213546-2213298 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT21878 NC_008769:c2213546-2213298 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References