Detailed information of TA system TA218772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3346170..3347890
• Replicon: chromosome
• Accession: NZ_CP084524
• Organism: Edwardsiella ictaluri strain S97-773

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: C5BBX2
• Locus tag: DB731_RS15810
• Protein ID: WP_015872652.1
• Coordinates: 3347234..3347890 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: C5BBX1
• Locus tag: DB731_RS15805
• Protein ID: WP_015872651.1
• Coordinates: 3346170..3347237 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DB731_RS15790 (DB731_15795)	3341410..3341964	+	555	WP_015872648.1	hypothetical protein	-
DB731_RS15795 (DB731_15800)	3342195..3342911	-	717	WP_015872649.1	SprT family zinc-dependent metalloprotease	-
DB731_RS15800 (DB731_15805)	3342911..3346006	-	3096	WP_015872650.1	HsdR family type I site-specific deoxyribonuclease	-
DB731_RS15805 (DB731_15810)	3346170..3347237	-	1068	WP_015872651.1	macro domain-containing protein	Antitoxin
DB731_RS15810 (DB731_15815)	3347234..3347890	-	657	WP_015872652.1	DUF4433 domain-containing protein	Toxin
DB731_RS15815 (DB731_15820)	3347903..3349219	-	1317	WP_226092363.1	restriction endonuclease subunit S	-
DB731_RS15820 (DB731_15825)	3349219..3351657	-	2439	WP_015872654.1	type I restriction-modification system subunit M	-
DB731_RS15825 (DB731_15830)	3351696..3351935	-	240	WP_015872655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3338526..3353544	15018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25178.69 Da
• Isoelectric Point: 6.9647

>T218772 WP_015872652.1 NZ_CP084524:c3347890-3347234 [Edwardsiella ictaluri]
MAYDYSANLNPEKALIWRIVHRDNIPWILDHGLHCGNSPVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRNVAAQNIPFVFTDSHAYYNWVNYYTRLDDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWKHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T218772 NZ_CP116100:c158380-158051 [Escherichia coli]
ATGTTTGATCACTGGTTTACCTCGCTCAACGATACCGACAGGGCCAGCGTGCTTGCGGCGCTGTTGGTCCTGCGGGAAAA
GGGGCCGGGGTTATCCAGACCTTATGCCGATACGATCAGAGGTTCCCGCTACAGCAACATGAAAGAGTTGCGTATTCAGA
GCCGGGGCGATCCGATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACTGGCATTGTGCTTTGTGCCGGAAATAAGGTC
GGCAATGAAAAACGTTTTTACGATGAGATGCTCCCGATCGCAGACCGGGAATTCACGAACTGGCTGAACACATTAAAAGA
GAAGGAGTAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40619.75 Da
• Isoelectric Point: 5.6965

>AT218772 WP_015872651.1 NZ_CP084524:c3347237-3346170 [Edwardsiella ictaluri]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYADACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT218772 NZ_CP116100:c158046-157744 [Escherichia coli]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAACCTGAAGTCGTGGACGAAGCTCAGGCGATGGCGACGGACAT
CCTGCTCAACATTCATCTTGCTGAGTTGCGTGAGAAAGTACAGAAAACGCAGGTGGAAATGGCGCAGGCGCTGGGTATCA
GACAGCCTACCGTTGCAGGAATGGAAAAACCGGGCCGCGACCTGAAACTTTCCACGCTTAAACGCTACGTTGAAGCGACC
GGCGGCAAATTACGCCTTGATGTTGAACTGCCGGATGGTTCTCACTACGGATTCGTACTGTAA

Structures

Toxin

• AlphaFold DB: C5BBX2

Antitoxin

• AlphaFold DB: C5BBX1