Detailed information of TA system TA218771

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3243333..3243996
• Replicon: chromosome
• Accession: NZ_CP084524
• Organism: Edwardsiella ictaluri strain S97-773

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: C5BAS3
• Locus tag: DB731_RS15345
• Protein ID: WP_015872556.1
• Coordinates: 3243333..3243749 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: C5BAS4
• Locus tag: DB731_RS15350
• Protein ID: WP_015872557.1
• Coordinates: 3243730..3243996 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DB731_RS15325 (DB731_15330)	3239257..3240990	-	1734	WP_015872552.1	single-stranded-DNA-specific exonuclease RecJ	-
DB731_RS15330 (DB731_15335)	3240995..3241711	-	717	WP_015872553.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
DB731_RS15335 (DB731_15340)	3241739..3242638	-	900	WP_015872554.1	site-specific tyrosine recombinase XerD	-
DB731_RS15340 (DB731_15345)	3242796..3243308	+	513	WP_015872555.1	flavodoxin FldB	-
DB731_RS15345 (DB731_15350)	3243333..3243749	-	417	WP_015872556.1	protein YgfX	Toxin
DB731_RS15350 (DB731_15355)	3243730..3243996	-	267	WP_015872557.1	FAD assembly factor SdhE	Antitoxin
DB731_RS15355 (DB731_15360)	3244344..3245339	+	996	WP_015872558.1	tRNA-modifying protein YgfZ	-
DB731_RS15360 (DB731_15365)	3245380..3246204	-	825	WP_015872559.1	shikimate 5-dehydrogenase	-
DB731_RS15365 (DB731_15370)	3246366..3247238	-	873	WP_015872560.1	nucleoside-specific channel-forming protein Tsx	-
DB731_RS15370 (DB731_15375)	3247847..3248497	+	651	WP_035609190.1	hemolysin III family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16145.89 Da
• Isoelectric Point: 11.3233

>T218771 WP_015872556.1 NZ_CP084524:c3243749-3243333 [Edwardsiella ictaluri]
VALWRCNVHASWRTQVMFLLGHGALVLAILLAPWPENYSVFWLILLVAVIFECIRSQRLIARNSGELQRLAPQVWHWQLR
EWRLARRPWVSDLGALLILQSTHGTPCRRRLWLAADSMSREEWGQLRRALLDTGDDRV

Nucleotide

• Length: 417 bp

>T218771 NZ_CP116100:109953-110207 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10364.92 Da
• Isoelectric Point: 5.6951

>AT218771 WP_015872557.1 NZ_CP084524:c3243996-3243730 [Edwardsiella ictaluri]
MDINNKARVHWACRRGMRELDIAIMPFFEHEYDALNDADKQTFVRLLSCDDPELFKWLMNQGKPDDTDLARMITLIQTRN
KERGPLAL

Nucleotide

• Length: 267 bp

>AT218771 NZ_CP116100:109705-109956 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: C5BAS3

Antitoxin

• AlphaFold DB: C5BAS4