Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1086717..1087309 Replicon chromosome
Accession NZ_CP084524
Organism Edwardsiella ictaluri strain S97-773

Toxin (Protein)


Gene name Hha Uniprot ID D0ZEC2
Locus tag DB731_RS05025 Protein ID WP_012847872.1
Coordinates 1086717..1086920 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID C5BCY7
Locus tag DB731_RS05030 Protein ID WP_015870487.1
Coordinates 1086941..1087309 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DB731_RS04985 (DB731_04975) 1081937..1082398 + 462 WP_015870480.1 Lrp/AsnC family transcriptional regulator -
DB731_RS04990 (DB731_04980) 1082427..1082594 - 168 WP_015870481.1 hypothetical protein -
DB731_RS04995 (DB731_04985) 1082697..1083035 + 339 WP_015870482.1 P-II family nitrogen regulator -
DB731_RS05000 (DB731_04990) 1083057..1084337 + 1281 WP_035610376.1 ammonium transporter AmtB -
DB731_RS05005 (DB731_04995) 1084371..1085237 - 867 WP_015870484.1 acyl-CoA thioesterase II -
DB731_RS05010 (DB731_05000) 1085283..1085612 - 330 WP_015870485.1 MGMT family protein -
DB731_RS05020 (DB731_05010) 1086590..1086697 + 108 Protein_967 IS5/IS1182 family transposase -
DB731_RS05025 (DB731_05015) 1086717..1086920 - 204 WP_012847872.1 HHA domain-containing protein Toxin
DB731_RS05030 (DB731_05020) 1086941..1087309 - 369 WP_015870487.1 Hha toxicity modulator TomB Antitoxin
DB731_RS05035 (DB731_05025) 1087669..1090821 - 3153 WP_015870488.1 efflux RND transporter permease subunit -
DB731_RS05040 (DB731_05030) 1090865..1092049 - 1185 WP_015870489.1 efflux RND transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8103.37 Da        Isoelectric Point: 5.0914

>T218768 WP_012847872.1 NZ_CP084524:c1086920-1086717 [Edwardsiella ictaluri]
MTKIDYLMKLRKCTTLDTLERVIEKNKYELSDDELEIFYSAADHRLAELTMNKLYDKIPAEVWQYVR

Download         Length: 204 bp

>T218768 NZ_CP116095:49836-50234 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14287.03 Da        Isoelectric Point: 5.4786

>AT218768 WP_015870487.1 NZ_CP084524:c1087309-1086941 [Edwardsiella ictaluri]
MDENTSYQHDISELKYLCDYLYHQGIDVLGESNHGWVSDPTAEVNLQLNELIEHIASIAQSFKIKYPRHSDLAEMLDYYL
DETYALFGTYSISETALRQWLRTKRRMAYCLAHEKRNAALHV

Download         Length: 369 bp

>AT218768 NZ_CP116095:49609-49836 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A034SMG4

Antitoxin

Source ID Structure
AlphaFold DB C5BCY7

References