Detailed information of TA system TA218766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3349573..3351293
• Replicon: chromosome
• Accession: NZ_CP084521
• Organism: Edwardsiella ictaluri strain S07-698

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: C5BBX2
• Locus tag: DB741_RS15840
• Protein ID: WP_015872652.1
• Coordinates: 3350637..3351293 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: C5BBX1
• Locus tag: DB741_RS15835
• Protein ID: WP_015872651.1
• Coordinates: 3349573..3350640 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DB741_RS15820 (DB741_15810)	3344813..3345367	+	555	WP_015872648.1	hypothetical protein	-
DB741_RS15825 (DB741_15815)	3345598..3346314	-	717	WP_015872649.1	SprT family zinc-dependent metalloprotease	-
DB741_RS15830 (DB741_15820)	3346314..3349409	-	3096	WP_015872650.1	HsdR family type I site-specific deoxyribonuclease	-
DB741_RS15835 (DB741_15825)	3349573..3350640	-	1068	WP_015872651.1	macro domain-containing protein	Antitoxin
DB741_RS15840 (DB741_15830)	3350637..3351293	-	657	WP_015872652.1	DUF4433 domain-containing protein	Toxin
DB741_RS15845 (DB741_15835)	3351306..3352622	-	1317	WP_226092363.1	restriction endonuclease subunit S	-
DB741_RS15850 (DB741_15840)	3352622..3355060	-	2439	WP_015872654.1	type I restriction-modification system subunit M	-
DB741_RS15855 (DB741_15845)	3355099..3355338	-	240	WP_015872655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3341929..3357319	15390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25178.69 Da
• Isoelectric Point: 6.9647

>T218766 WP_015872652.1 NZ_CP084521:c3351293-3350637 [Edwardsiella ictaluri]
MAYDYSANLNPEKALIWRIVHRDNIPWILDHGLHCGNSPVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRNVAAQNIPFVFTDSHAYYNWVNYYTRLDDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWKHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T218766 NZ_CP116094:c4528117-4528010 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40619.75 Da
• Isoelectric Point: 5.6965

>AT218766 WP_015872651.1 NZ_CP084521:c3350640-3349573 [Edwardsiella ictaluri]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYADACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT218766 NZ_CP116094:4528165-4528231 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: C5BBX2

Antitoxin

• AlphaFold DB: C5BBX1