Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1089828..1090420 Replicon chromosome
Accession NZ_CP084521
Organism Edwardsiella ictaluri strain S07-698

Toxin (Protein)


Gene name Hha Uniprot ID D0ZEC2
Locus tag DB741_RS05030 Protein ID WP_012847872.1
Coordinates 1089828..1090031 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID C5BCY7
Locus tag DB741_RS05035 Protein ID WP_015870487.1
Coordinates 1090052..1090420 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DB741_RS04990 (DB741_04985) 1085048..1085509 + 462 WP_015870480.1 Lrp/AsnC family transcriptional regulator -
DB741_RS04995 (DB741_04990) 1085538..1085705 - 168 WP_015870481.1 hypothetical protein -
DB741_RS05000 (DB741_04995) 1085808..1086146 + 339 WP_015870482.1 P-II family nitrogen regulator -
DB741_RS05005 (DB741_05000) 1086168..1087448 + 1281 WP_035610376.1 ammonium transporter AmtB -
DB741_RS05010 (DB741_05005) 1087482..1088348 - 867 WP_015870484.1 acyl-CoA thioesterase II -
DB741_RS05015 (DB741_05010) 1088394..1088723 - 330 WP_015870485.1 MGMT family protein -
DB741_RS05025 (DB741_05020) 1089701..1089808 + 108 Protein_968 IS5/IS1182 family transposase -
DB741_RS05030 (DB741_05025) 1089828..1090031 - 204 WP_012847872.1 HHA domain-containing protein Toxin
DB741_RS05035 (DB741_05030) 1090052..1090420 - 369 WP_015870487.1 Hha toxicity modulator TomB Antitoxin
DB741_RS05040 (DB741_05035) 1090780..1093932 - 3153 WP_015870488.1 efflux RND transporter permease subunit -
DB741_RS05045 (DB741_05040) 1093976..1095160 - 1185 WP_015870489.1 efflux RND transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8103.37 Da        Isoelectric Point: 5.0914

>T218762 WP_012847872.1 NZ_CP084521:c1090031-1089828 [Edwardsiella ictaluri]
MTKIDYLMKLRKCTTLDTLERVIEKNKYELSDDELEIFYSAADHRLAELTMNKLYDKIPAEVWQYVR

Download         Length: 204 bp

>T218762 NZ_CP116094:3915981-3916358 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14287.03 Da        Isoelectric Point: 5.4786

>AT218762 WP_015870487.1 NZ_CP084521:c1090420-1090052 [Edwardsiella ictaluri]
MDENTSYQHDISELKYLCDYLYHQGIDVLGESNHGWVSDPTAEVNLQLNELIEHIASIAQSFKIKYPRHSDLAEMLDYYL
DETYALFGTYSISETALRQWLRTKRRMAYCLAHEKRNAALHV

Download         Length: 369 bp

>AT218762 NZ_CP116094:3915524-3915892 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A034SMG4

Antitoxin

Source ID Structure
AlphaFold DB C5BCY7

References