Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 66994..67637 Replicon plasmid pIncFIIK_FIA
Accession NZ_CP084504
Organism Klebsiella pneumoniae strain MI01_K

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag KRU50_RS27085 Protein ID WP_048983057.1
Coordinates 67221..67637 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag KRU50_RS27080 Protein ID WP_016338373.1
Coordinates 66994..67224 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRU50_RS27055 (KRU50_27085) 62413..62601 + 189 Protein_65 IS3 family transposase -
KRU50_RS27060 (KRU50_27090) 62863..63159 - 297 WP_001542733.1 helix-turn-helix transcriptional regulator -
KRU50_RS27065 (KRU50_27095) 63489..64457 + 969 WP_077265821.1 IS5 family transposase -
KRU50_RS27070 (KRU50_27100) 64655..65323 + 669 WP_032249507.1 LuxR C-terminal-related transcriptional regulator -
KRU50_RS27075 (KRU50_27105) 65369..66349 + 981 WP_000019403.1 IS5-like element IS5 family transposase -
KRU50_RS27080 (KRU50_27110) 66994..67224 + 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KRU50_RS27085 (KRU50_27115) 67221..67637 + 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
KRU50_RS27090 (KRU50_27120) 67678..68001 - 324 WP_085842301.1 hypothetical protein -
KRU50_RS27095 (KRU50_27125) 68019..68555 - 537 WP_223825639.1 hypothetical protein -
KRU50_RS27100 (KRU50_27130) 69214..70491 + 1278 WP_016338369.1 HlyD family secretion protein -
KRU50_RS27105 (KRU50_27135) 70481..71686 + 1206 Protein_75 cysteine peptidase family C39 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrB1 - 1..149924 149924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T218742 WP_048983057.1 NZ_CP084504:67221-67637 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T218742 NZ_CP116089:142907-143305 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT218742 WP_016338373.1 NZ_CP084504:66994-67224 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT218742 NZ_CP116089:142680-142907 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References