Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 46320..47056 Replicon plasmid pIncFIIK_FIA
Accession NZ_CP084504
Organism Klebsiella pneumoniae strain MI01_K

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KRU50_RS27005 Protein ID WP_003026803.1
Coordinates 46574..47056 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KRU50_RS27000 Protein ID WP_003026799.1
Coordinates 46320..46586 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRU50_RS26980 (KRU50_27010) 42528..43019 + 492 WP_032249453.1 hypothetical protein -
KRU50_RS26985 (KRU50_27015) 44244..44594 - 351 WP_004200891.1 hypothetical protein -
KRU50_RS26990 (KRU50_27020) 45046..45594 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
KRU50_RS26995 (KRU50_27025) 45641..46075 + 435 WP_001567367.1 cell envelope integrity protein TolA -
KRU50_RS27000 (KRU50_27030) 46320..46586 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KRU50_RS27005 (KRU50_27035) 46574..47056 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KRU50_RS27010 (KRU50_27040) 47437..47853 + 417 WP_077265827.1 hypothetical protein -
KRU50_RS27015 (KRU50_27045) 47822..48990 + 1169 WP_085929673.1 IS3-like element ISEc52 family transposase -
KRU50_RS27020 (KRU50_27050) 49191..49994 + 804 WP_000619352.1 TIGR02391 family protein -
KRU50_RS27025 (KRU50_27055) 50015..51448 + 1434 WP_004016055.1 restriction endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrB1 - 1..149924 149924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T218741 WP_003026803.1 NZ_CP084504:46574-47056 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T218741 NZ_CP116089:c133951-133802 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT218741 WP_003026799.1 NZ_CP084504:46320-46586 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT218741 NZ_CP116089:133995-134056 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References