Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5167119..5167744 Replicon chromosome
Accession NZ_CP084502
Organism Klebsiella pneumoniae strain MI01_K

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag KRU50_RS24970 Protein ID WP_019705794.1
Coordinates 5167119..5167502 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KRU50_RS24975 Protein ID WP_004150355.1
Coordinates 5167502..5167744 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRU50_RS24955 (5164485) 5164485..5165387 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KRU50_RS24960 (5165384) 5165384..5166019 + 636 WP_032430299.1 formate dehydrogenase cytochrome b556 subunit -
KRU50_RS24965 (5166016) 5166016..5166945 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KRU50_RS24970 (5167119) 5167119..5167502 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
KRU50_RS24975 (5167502) 5167502..5167744 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KRU50_RS24980 (5167949) 5167949..5168866 + 918 WP_032430300.1 alpha/beta hydrolase -
KRU50_RS24985 (5168881) 5168881..5169822 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KRU50_RS24990 (5169867) 5169867..5170304 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KRU50_RS24995 (5170301) 5170301..5171161 - 861 WP_004146232.1 virulence factor BrkB family protein -
KRU50_RS25000 (5171155) 5171155..5171754 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T218738 WP_019705794.1 NZ_CP084502:c5167502-5167119 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T218738 NZ_CP116089:c3194-2889 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT218738 WP_004150355.1 NZ_CP084502:c5167744-5167502 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT218738 NZ_CP116089:c3414-3196 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References