Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3963630..3964249 Replicon chromosome
Accession NZ_CP084502
Organism Klebsiella pneumoniae strain MI01_K

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KRU50_RS19230 Protein ID WP_002892050.1
Coordinates 3964031..3964249 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KRU50_RS19225 Protein ID WP_002892066.1
Coordinates 3963630..3964004 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KRU50_RS19215 (3958782) 3958782..3959975 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KRU50_RS19220 (3959998) 3959998..3963144 + 3147 WP_004147373.1 multidrug efflux RND transporter permease subunit AcrB -
KRU50_RS19225 (3963630) 3963630..3964004 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KRU50_RS19230 (3964031) 3964031..3964249 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KRU50_RS19235 (3964408) 3964408..3964974 + 567 WP_002892030.1 maltose O-acetyltransferase -
KRU50_RS19240 (3964946) 3964946..3965086 - 141 WP_004147370.1 hypothetical protein -
KRU50_RS19245 (3965107) 3965107..3965577 + 471 WP_002892026.1 YlaC family protein -
KRU50_RS19250 (3965552) 3965552..3967003 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KRU50_RS19255 (3967104) 3967104..3967802 + 699 WP_040220001.1 GNAT family protein -
KRU50_RS19260 (3967799) 3967799..3967939 - 141 WP_040219999.1 type B 50S ribosomal protein L36 -
KRU50_RS19265 (3967939) 3967939..3968202 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T218734 WP_002892050.1 NZ_CP084502:3964031-3964249 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T218734 NZ_CP116088:3351232-3351543 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT218734 WP_002892066.1 NZ_CP084502:3963630-3964004 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT218734 NZ_CP116088:3351544-3351834 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References