Detailed information of TA system TA218733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2634838..2636426
• Replicon: chromosome
• Accession: NZ_CP084502
• Organism: Klebsiella pneumoniae strain MI01_K

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A6T928
• Locus tag: KRU50_RS12875
• Protein ID: WP_004176197.1
• Coordinates: 2634838..2636160 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KRU50_RS12880
• Protein ID: WP_032417111.1
• Coordinates: 2636160..2636426 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRU50_RS12860 (2629894)	2629894..2632395	+	2502	WP_016831617.1	fimbria/pilus outer membrane usher protein	-
KRU50_RS12865 (2632383)	2632383..2633387	+	1005	WP_004176196.1	fimbrial protein	-
KRU50_RS12870 (2633529)	2633529..2634821	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KRU50_RS12875 (2634838)	2634838..2636160	-	1323	WP_004176197.1	type II toxin-antitoxin system HipA family toxin	Toxin
KRU50_RS12880 (2636160)	2636160..2636426	-	267	WP_032417111.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KRU50_RS12885 (2636691)	2636691..2637017	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
KRU50_RS12890 (2637014)	2637014..2637514	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KRU50_RS12895 (2637492)	2637492..2638871	-	1380	WP_021440115.1	nucleobase:cation symporter-2 family protein	-
KRU50_RS12900 (2639217)	2639217..2640371	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KRU50_RS12905 (2640352)	2640352..2641275	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49111.41 Da
• Isoelectric Point: 7.5357

>T218733 WP_004176197.1 NZ_CP084502:c2636160-2634838 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T218733 NZ_CP116088:c2879245-2879015 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9926.32 Da
• Isoelectric Point: 8.5912

>AT218733 WP_032417111.1 NZ_CP084502:c2636426-2636160 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETASAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT218733 NZ_CP116088:c2879018-2878659 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A6T928