Detailed information of TA system TA218720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 723..1197
• Replicon: plasmid p1632-4
• Accession: NZ_CP084501
• Organism: Klebsiella pneumoniae strain 1632

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: LDL62_RS28900
• Protein ID: WP_004197762.1
• Coordinates: 931..1197 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: LDL62_RS28895
• Protein ID: WP_000879771.1
• Coordinates: 723..947 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL62_RS28895 (LDL62_28895)	723..947	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
LDL62_RS28900 (LDL62_28900)	931..1197	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDL62_RS28905 (LDL62_28905)	1267..1617	-	351	WP_004197756.1	hypothetical protein	-
LDL62_RS28910 (LDL62_28910)	1683..2228	-	546	WP_023317533.1	hypothetical protein	-
LDL62_RS28915 (LDL62_28915)	2254..2580	-	327	WP_050516389.1	hypothetical protein	-
LDL62_RS28920 (LDL62_28920)	3736..3912	+	177	WP_004197757.1	hypothetical protein	-
LDL62_RS28925 (LDL62_28925)	3902..4246	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
LDL62_RS29000	4455..4889	+	435	Protein_8	relaxase/mobilization nuclease domain-containing protein	-
LDL62_RS28935 (LDL62_28935)	4924..5433	+	510	WP_032433972.1	MbeB family mobilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T218720 WP_004197762.1 NZ_CP084501:931-1197 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T218720 NZ_CP116088:114255-114506 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT218720 WP_000879771.1 NZ_CP084501:723-947 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT218720 NZ_CP116088:114503-114757 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5