Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1952246..1952859 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag BCG_RS09085 Protein ID WP_003408465.1
Coordinates 1952246..1952635 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag BCG_RS09090 Protein ID WP_003408469.1
Coordinates 1952632..1952859 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS09050 1947876..1948790 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
BCG_RS09055 1948793..1949623 + 831 WP_003408448.1 cyclase family protein -
BCG_RS09060 1949623..1949973 + 351 WP_003898986.1 cupin domain-containing protein -
BCG_RS09065 1950026..1950843 + 818 Protein_1771 3-keto-5-aminohexanoate cleavage protein -
BCG_RS09070 1950857..1951636 + 780 WP_003408460.1 IclR family transcriptional regulator -
BCG_RS09085 1952246..1952635 - 390 WP_003408465.1 PIN domain-containing protein Toxin
BCG_RS09090 1952632..1952859 - 228 WP_003408469.1 antitoxin Antitoxin
BCG_RS09095 1953077..1954561 + 1485 WP_010950580.1 biotin carboxylase -
BCG_RS09100 1954558..1955805 + 1248 WP_003408476.1 serine hydrolase -
BCG_RS09105 1955848..1956267 - 420 WP_003408483.1 hypothetical protein -
BCG_RS09110 1956257..1956967 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T21872 WP_003408465.1 NC_008769:c1952635-1952246 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T21872 NC_008769:c1952635-1952246 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT21872 WP_003408469.1 NC_008769:c1952859-1952632 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT21872 NC_008769:c1952859-1952632 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References