Detailed information of TA system TA218719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 7053..7633
• Replicon: plasmid p1632-3
• Accession: NZ_CP084500
• Organism: Klebsiella pneumoniae strain 1632

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LDL62_RS28865
• Protein ID: WP_071177730.1
• Coordinates: 7319..7633 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LDL62_RS28860
• Protein ID: WP_000093040.1
• Coordinates: 7053..7331 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL62_RS28830 (LDL62_28830)	3113..3262	-	150	WP_032440454.1	colicin release lysis protein	-
LDL62_RS28835 (LDL62_28835)	3347..3604	-	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
LDL62_RS28840 (LDL62_28840)	3614..5299	-	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
LDL62_RS28845 (LDL62_28845)	5626..5871	+	246	WP_032440458.1	hypothetical protein	-
LDL62_RS28850 (LDL62_28850)	6145..6516	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
LDL62_RS28855 (LDL62_28855)	6513..6878	+	366	WP_072354022.1	TonB family protein	-
LDL62_RS28860 (LDL62_28860)	7053..7331	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LDL62_RS28865 (LDL62_28865)	7319..7633	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDL62_RS28870 (LDL62_28870)	7797..8225	+	429	WP_001140599.1	hypothetical protein	-
LDL62_RS28875 (LDL62_28875)	8251..8430	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LDL62_RS28880 (LDL62_28880)	8457..8987	-	531	WP_071177729.1	hypothetical protein	-
LDL62_RS28885 (LDL62_28885)	8994..9725	-	732	WP_071177728.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T218719 WP_071177730.1 NZ_CP084500:7319-7633 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T218719 NZ_CP116087:12095-12400 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT218719 WP_000093040.1 NZ_CP084500:7053-7331 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT218719 NZ_CP116087:11875-12093 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1