218712

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5402495..5403120 Replicon chromosome
Accession NZ_CP084497
Organism Klebsiella pneumoniae strain 1632

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LDL62_RS26795 Protein ID WP_002882817.1
Coordinates 5402495..5402878 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LDL62_RS26800 Protein ID WP_004150355.1
Coordinates 5402878..5403120 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDL62_RS26780 (LDL62_26780) 5399861..5400763 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LDL62_RS26785 (LDL62_26785) 5400760..5401395 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LDL62_RS26790 (LDL62_26790) 5401392..5402321 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LDL62_RS26795 (LDL62_26795) 5402495..5402878 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LDL62_RS26800 (LDL62_26800) 5402878..5403120 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LDL62_RS26805 (LDL62_26805) 5403325..5404242 + 918 WP_002882812.1 alpha/beta hydrolase -
LDL62_RS26810 (LDL62_26810) 5404256..5405197 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LDL62_RS26815 (LDL62_26815) 5405242..5405679 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LDL62_RS26820 (LDL62_26820) 5405676..5406536 - 861 WP_002882807.1 virulence factor BrkB family protein -
LDL62_RS26825 (LDL62_26825) 5406530..5407129 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T218712 WP_002882817.1 NZ_CP084497:c5402878-5402495 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T218712 NZ_CP116085:3669263-3669481 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT218712 WP_004150355.1 NZ_CP084497:c5403120-5402878 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT218712 NZ_CP116085:3668863-3669237 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References