21870

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1711344..1711960 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag BCG_RS08075 Protein ID WP_003407593.1
Coordinates 1711643..1711960 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7U4BU54
Locus tag BCG_RS08070 Protein ID WP_011799188.1
Coordinates 1711344..1711646 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS08060 1707230..1709077 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
BCG_RS08065 1709078..1711330 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
BCG_RS08070 1711344..1711646 + 303 WP_011799188.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
BCG_RS08075 1711643..1711960 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
BCG_RS08080 1711957..1712961 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
BCG_RS08085 1713014..1714303 + 1290 WP_003407599.1 serine hydrolase -
BCG_RS08090 1714376..1715149 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
BCG_RS08095 1715207..1715398 - 192 WP_003407609.1 dodecin family protein -
BCG_RS22235 1715429..1715731 - 303 WP_003407610.1 NUDIX domain-containing protein -
BCG_RS08105 1715923..1716951 + 1029 WP_011799189.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T21870 WP_003407593.1 NC_008769:1711643-1711960 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T21870 NC_008769:1711643-1711960 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11199.50 Da        Isoelectric Point: 9.5564

>AT21870 WP_011799188.1 NC_008769:1711344-1711646 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
VPFLVALSGIILGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT21870 NC_008769:1711344-1711646 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTTGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BU54

References