Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 81017..81660 Replicon plasmid p1864-2
Accession NZ_CP084494
Organism Klebsiella pneumoniae strain 1864

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LDL64_RS28755 Protein ID WP_001044770.1
Coordinates 81017..81433 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LDL64_RS28760 Protein ID WP_001261282.1
Coordinates 81430..81660 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDL64_RS28735 (77120) 77120..77392 - 273 Protein_102 transposase -
LDL64_RS28745 (78374) 78374..79396 - 1023 WP_000361404.1 helicase UvrD -
LDL64_RS28750 (79381) 79381..80943 - 1563 WP_004206609.1 AAA family ATPase -
LDL64_RS28755 (81017) 81017..81433 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LDL64_RS28760 (81430) 81430..81660 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LDL64_RS28765 (81617) 81617..82078 + 462 WP_014343465.1 hypothetical protein -
LDL64_RS28770 (82239) 82239..83183 + 945 WP_011977810.1 hypothetical protein -
LDL64_RS28775 (83220) 83220..83612 + 393 WP_011977811.1 hypothetical protein -
LDL64_RS28780 (83670) 83670..84191 + 522 WP_013214008.1 hypothetical protein -
LDL64_RS28785 (84237) 84237..84440 + 204 WP_011977813.1 hypothetical protein -
LDL64_RS28790 (84470) 84470..85474 + 1005 WP_011977814.1 hypothetical protein -
LDL64_RS28795 (85658) 85658..86437 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..152725 152725


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T218691 WP_001044770.1 NZ_CP084494:c81433-81017 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T218691 NZ_CP116076:2666-2944 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT218691 WP_001261282.1 NZ_CP084494:c81660-81430 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT218691 NZ_CP116076:2397-2666 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGGGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References