Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1598900..1599555 Replicon chromosome
Accession NC_008769
Organism Mycobacterium bovis BCG str. Pasteur 1173P2

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag BCG_RS07560 Protein ID WP_003407268.1
Coordinates 1598900..1599301 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag BCG_RS07565 Protein ID WP_003407272.1
Coordinates 1599298..1599555 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG_RS07545 1594744..1596129 - 1386 WP_003407260.1 cytochrome P450 -
BCG_RS07550 1596207..1597241 + 1035 WP_003407264.1 AraC family transcriptional regulator -
BCG_RS07555 1597287..1598645 - 1359 WP_010950541.1 PE family protein -
BCG_RS07560 1598900..1599301 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
BCG_RS07565 1599298..1599555 - 258 WP_003407272.1 ribbon-helix-helix domain-containing protein Antitoxin
BCG_RS07570 1599638..1600597 - 960 WP_011799179.1 alpha/beta hydrolase -
BCG_RS07575 1600622..1601584 - 963 WP_003407279.1 alpha/beta hydrolase -
BCG_RS07580 1601583..1602320 + 738 WP_031647000.1 lysoplasmalogenase -
BCG_RS07585 1602401..1604368 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T21869 WP_003407268.1 NC_008769:c1599301-1598900 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T21869 NC_008769:c1599301-1598900 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT21869 WP_003407272.1 NC_008769:c1599555-1599298 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT21869 NC_008769:c1599555-1599298 [Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References