Detailed information of TA system TA218687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 214311..214833
• Replicon: plasmid p1864-1
• Accession: NZ_CP084493
• Organism: Klebsiella pneumoniae strain 1864

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: LDL64_RS27805
• Protein ID: WP_004181778.1
• Coordinates: 214549..214833 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: LDL64_RS27800
• Protein ID: WP_004181777.1
• Coordinates: 214311..214559 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL64_RS27780 (LDL64_27780)	209520..210356	-	837	WP_014386523.1	hypothetical protein	-
LDL64_RS27785 (LDL64_27785)	210403..210756	-	354	WP_004181774.1	hypothetical protein	-
LDL64_RS27790 (LDL64_27790)	210901..211887	-	987	WP_101992392.1	hypothetical protein	-
LDL64_RS27795 (LDL64_27795)	212221..214020	-	1800	WP_004181776.1	ATP-dependent helicase	-
LDL64_RS27800 (LDL64_27800)	214311..214559	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LDL64_RS27805 (LDL64_27805)	214549..214833	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LDL64_RS27810 (LDL64_27810)	214850..214951	-	102	Protein_234	IS200/IS605 family transposase	-
LDL64_RS27815 (LDL64_27815)	214987..216213	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
LDL64_RS27820 (LDL64_27820)	216484..216708	-	225	Protein_236	transposase	-
LDL64_RS27825 (LDL64_27825)	216787..217215	-	429	WP_077257669.1	IS200/IS605 family transposase	-
LDL64_RS27830 (LDL64_27830)	217251..218438	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-
LDL64_RS27835 (LDL64_27835)	218483..218836	-	354	WP_226034509.1	hypothetical protein	-
LDL64_RS27840 (LDL64_27840)	218851..219243	-	393	WP_226034510.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4	iucA / iucB / iucC / iucD / iutA / rmpA	1..296908	296908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T218687 WP_004181778.1 NZ_CP084493:214549-214833 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T218687 NZ_CP116074:3653063-3653281 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT218687 WP_004181777.1 NZ_CP084493:214311-214559 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT218687 NZ_CP116074:3652663-3653037 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8