Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5299392..5300017 Replicon chromosome
Accession NZ_CP084492
Organism Klebsiella pneumoniae strain 1864

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LDL64_RS26265 Protein ID WP_002882817.1
Coordinates 5299392..5299775 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LDL64_RS26270 Protein ID WP_004150355.1
Coordinates 5299775..5300017 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDL64_RS26250 (LDL64_26250) 5296758..5297660 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LDL64_RS26255 (LDL64_26255) 5297657..5298292 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LDL64_RS26260 (LDL64_26260) 5298289..5299218 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LDL64_RS26265 (LDL64_26265) 5299392..5299775 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LDL64_RS26270 (LDL64_26270) 5299775..5300017 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LDL64_RS26275 (LDL64_26275) 5300222..5301139 + 918 WP_002882812.1 alpha/beta hydrolase -
LDL64_RS26280 (LDL64_26280) 5301153..5302094 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LDL64_RS26285 (LDL64_26285) 5302139..5302576 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LDL64_RS26290 (LDL64_26290) 5302573..5303433 - 861 WP_002882807.1 virulence factor BrkB family protein -
LDL64_RS26295 (LDL64_26295) 5303427..5304026 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T218683 WP_002882817.1 NZ_CP084492:c5299775-5299392 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T218683 NZ_CP116074:2776864-2776971 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT218683 WP_004150355.1 NZ_CP084492:c5300017-5299775 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT218683 NZ_CP116074:c2776811-2776750 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References