Detailed information of TA system TA218682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4975341..4977022
• Replicon: chromosome
• Accession: NZ_CP084492
• Organism: Klebsiella pneumoniae strain 1864

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: LDL64_RS24750
• Protein ID: WP_002885155.1
• Coordinates: 4975341..4976258 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: W8UPQ8
• Locus tag: LDL64_RS24755
• Protein ID: WP_004151732.1
• Coordinates: 4976264..4977022 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL64_RS24730 (LDL64_24730)	4970647..4972167	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
LDL64_RS24735 (LDL64_24735)	4972164..4973162	+	999	WP_002885172.1	ribose ABC transporter permease	-
LDL64_RS24740 (LDL64_24740)	4973193..4974134	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
LDL64_RS24745 (LDL64_24745)	4974209..4975216	+	1008	WP_004151731.1	carbohydrate kinase family protein	-
LDL64_RS24750 (LDL64_24750)	4975341..4976258	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LDL64_RS24755 (LDL64_24755)	4976264..4977022	-	759	WP_004151732.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
LDL64_RS24760 (LDL64_24760)	4977108..4977836	-	729	WP_002885152.1	response regulator transcription factor	-
LDL64_RS24765 (LDL64_24765)	4978109..4980256	+	2148	WP_077598858.1	formate dehydrogenase subunit alpha	-
LDL64_RS24770 (LDL64_24770)	4980486..4981172	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T218682 WP_002885155.1 NZ_CP084492:c4976258-4975341 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T218682 NZ_CP116074:c2600503-2600327 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28564.92 Da
• Isoelectric Point: 10.0870

>AT218682 WP_004151732.1 NZ_CP084492:c4977022-4976264 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLSFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT218682 NZ_CP116074:c2600281-2599865 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A0A0H3GQB5