Detailed information of TA system TA218665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 886016..886656
• Replicon: plasmid p-2
• Accession: NZ_CP084488
• Organism: Ensifer adhaerens strain E-60

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LDL63_RS31230
• Protein ID: WP_218685512.1
• Coordinates: 886270..886656 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1G9VC88
• Locus tag: LDL63_RS31225
• Protein ID: WP_034798574.1
• Coordinates: 886016..886270 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDL63_RS31195 (LDL63_31195)	881830..882915	-	1086	WP_060605049.1	isocitrate/isopropylmalate family dehydrogenase	-
LDL63_RS31200 (LDL63_31200)	883129..884043	+	915	WP_060604966.1	GntR family transcriptional regulator	-
LDL63_RS31205 (LDL63_31205)	884197..884643	-	447	Protein_795	multicopper oxidase domain-containing protein	-
LDL63_RS31210 (LDL63_31210)	884653..884943	-	291	WP_060521288.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LDL63_RS31215 (LDL63_31215)	884940..885188	-	249	WP_060521339.1	CopG family ribbon-helix-helix protein	-
LDL63_RS31220 (LDL63_31220)	885316..885467	-	152	Protein_798	PIN domain-containing protein	-
LDL63_RS31225 (LDL63_31225)	886016..886270	+	255	WP_034798574.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LDL63_RS31230 (LDL63_31230)	886270..886656	+	387	WP_218685512.1	type II toxin-antitoxin system VapC family toxin	Toxin
LDL63_RS31235 (LDL63_31235)	886858..887007	+	150	Protein_801	integrase	-
LDL63_RS31240 (LDL63_31240)	888210..888992	+	783	WP_060529962.1	GntR family transcriptional regulator	-
LDL63_RS31245 (LDL63_31245)	889021..890136	+	1116	WP_218685511.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
LDL63_RS31250 (LDL63_31250)	890153..891073	+	921	WP_218685527.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1568420	1568420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13908.70 Da
• Isoelectric Point: 4.4937

>T218665 WP_218685512.1 NZ_CP084488:886270-886656 [Ensifer adhaerens]
MVIDTSVLAAIAFNEPEAAAFRERIADDPVRLISAATVLEAAMVIETRLGEAAGADLDLWLYKADVEIVPVTAEHSDRAR
RAWRRYGKGRHPASLNYGDCFSYALAALSGEPLLYKGNDLSQTDIEAA

Nucleotide

• Length: 387 bp

>T218665 NZ_CP116071:c4395900-4395523 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9501.70 Da
• Isoelectric Point: 5.1864

>AT218665 WP_034798574.1 NZ_CP084488:886016-886270 [Ensifer adhaerens]
MALSIKDHDTEQLARTLAERTGESITLATKRALEERLRRLGSDLRKAALLEDLEAIQRRWNARPVLDSRTADEILGYDEN
GLPS

Nucleotide

• Length: 255 bp

>AT218665 NZ_CP116071:c4396358-4395990 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1G9VC88