Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-Xre
Location 279219..280099 Replicon plasmid p-1
Accession NZ_CP084487
Organism Ensifer adhaerens strain E-60

Toxin (Protein)


Gene name - Uniprot ID A0A1G9YN53
Locus tag LDL63_RS21020 Protein ID WP_060529103.1
Coordinates 279590..280099 (+) Length 170 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1G9YN41
Locus tag LDL63_RS21015 Protein ID WP_060529162.1
Coordinates 279219..279590 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDL63_RS20995 (LDL63_20995) 275112..275495 + 384 WP_060529107.1 RidA family protein -
LDL63_RS21000 (LDL63_21000) 275511..277499 + 1989 WP_060529106.1 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha -
LDL63_RS21005 (LDL63_21005) 277496..278365 + 870 WP_113504331.1 hydroxymethylglutaryl-CoA lyase -
LDL63_RS21010 (LDL63_21010) 278362..279147 + 786 WP_192443345.1 crotonase/enoyl-CoA hydratase family protein -
LDL63_RS21015 (LDL63_21015) 279219..279590 + 372 WP_060529162.1 DUF2384 domain-containing protein Antitoxin
LDL63_RS21020 (LDL63_21020) 279590..280099 + 510 WP_060529103.1 RES domain-containing protein Toxin
LDL63_RS21025 (LDL63_21025) 280132..281496 - 1365 WP_218686250.1 FAD-binding oxidoreductase -
LDL63_RS21030 (LDL63_21030) 281658..282782 - 1125 WP_064817026.1 sulfate/molybdate ABC transporter ATP-binding protein -
LDL63_RS21035 (LDL63_21035) 282814..283734 - 921 WP_112773461.1 sulfate ABC transporter permease subunit CysW -
LDL63_RS21040 (LDL63_21040) 283724..284590 - 867 WP_218686263.1 sulfate ABC transporter permease subunit CysT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA 1..1681071 1681071


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(8-162)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(71-120)


Sequences

Toxin        


Download         Length: 170 a.a.        Molecular weight: 18727.30 Da        Isoelectric Point: 6.3310

>T218663 WP_060529103.1 NZ_CP084487:279590-280099 [Ensifer adhaerens]
MAVGAPVRLWRAYVPRWAHAPLSGEGAGRYGGRWNPVGTPTIYAARELSTAWAEYNQGFVQHPALIAQLELRFARLADLT
APEILSDLGLTEEIHRCEWRDALDRGLVPETHRLQLALSDRGFDGLIYPSFMSPGGTCVALWHWNAGEGSELKVIDPEGR
LPKTPASWL

Download         Length: 510 bp

>T218663 NZ_CP116071:c3987444-3987046 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 12900.86 Da        Isoelectric Point: 9.8060

>AT218663 WP_060529162.1 NZ_CP084487:279219-279590 [Ensifer adhaerens]
MAAVGLNISTAHYGESHSSFLSARLVADRLGVTLAELAKLIGVARNTLTAKSSARKVDAALSSVVRILAMAGEMAGDDSR
AVIWFKHQPIPGWAGKTAYDLVGEGKADKVLAYLEAVRSGVYA

Download         Length: 372 bp

>AT218663 NZ_CP116071:c3987740-3987447 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G9YN53

Antitoxin

Source ID Structure
AlphaFold DB A0A1G9YN41

References