Detailed information of TA system TA218662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6200773..6201278
• Replicon: chromosome
• Accession: NZ_CP084484
• Organism: Pseudomonas sp. PS1(2021)

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LE197_RS29500
• Protein ID: WP_003083773.1
• Coordinates: 6200773..6201054 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LE197_RS29505
• Protein ID: WP_003083775.1
• Coordinates: 6201051..6201278 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LE197_RS29475 (LE197_29470)	6196024..6197373	+	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-
LE197_RS29480 (LE197_29475)	6197422..6198108	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LE197_RS29485 (LE197_29480)	6198209..6198943	+	735	WP_003117208.1	GntR family transcriptional regulator	-
LE197_RS29490 (LE197_29485)	6199123..6199533	+	411	WP_003110659.1	aegerolysin family protein	-
LE197_RS29495 (LE197_29490)	6199565..6200473	-	909	WP_057386940.1	LysR family transcriptional regulator	-
LE197_RS29500 (LE197_29495)	6200773..6201054	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LE197_RS29505 (LE197_29500)	6201051..6201278	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LE197_RS29510 (LE197_29505)	6201454..6202074	-	621	WP_003101226.1	hypothetical protein	-
LE197_RS29515 (LE197_29510)	6202175..6202675	+	501	WP_057386938.1	LEA type 2 family protein	-
LE197_RS29520 (LE197_29515)	6202748..6203089	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LE197_RS29525 (LE197_29520)	6203171..6204598	-	1428	WP_003083784.1	GABA permease	-
LE197_RS29530 (LE197_29525)	6204767..6206260	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T218662 WP_003083773.1 NZ_CP084484:c6201054-6200773 [Pseudomonas sp. PS1(2021)]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T218662 NZ_CP116071:3787935-3788153 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT218662 WP_003083775.1 NZ_CP084484:c6201278-6201051 [Pseudomonas sp. PS1(2021)]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT218662 NZ_CP116071:3787535-3787909 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9