Detailed information of TA system TA218661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 5751618..5752149
• Replicon: chromosome
• Accession: NZ_CP084484
• Organism: Pseudomonas sp. PS1(2021)

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LE197_RS27385
• Protein ID: WP_180690175.1
• Coordinates: 5751618..5751899 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: LE197_RS27390
• Protein ID: WP_180690176.1
• Coordinates: 5751886..5752149 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LE197_RS27370 (LE197_27365)	5747681..5749993	+	2313	WP_226033766.1	TonB-dependent receptor	-
LE197_RS27375 (LE197_27370)	5749968..5751059	+	1092	Protein_5426	methyl-accepting chemotaxis protein	-
LE197_RS27380 (LE197_27375)	5751149..5751397	-	249	Protein_5427	hypothetical protein	-
LE197_RS27385 (LE197_27380)	5751618..5751899	-	282	WP_180690175.1	type II toxin-antitoxin system YafQ family toxin	Toxin
LE197_RS27390 (LE197_27385)	5751886..5752149	-	264	WP_180690176.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LE197_RS27395 (LE197_27390)	5752421..5752957	-	537	WP_003159187.1	hypothetical protein	-
LE197_RS27400 (LE197_27395)	5752969..5753364	-	396	WP_003159186.1	type II toxin-antitoxin system VapC family toxin	-
LE197_RS27405 (LE197_27400)	5753361..5753612	-	252	WP_003108276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LE197_RS27410 (LE197_27405)	5753794..5754360	+	567	WP_226033767.1	recombinase family protein	-
LE197_RS27415 (LE197_27410)	5754502..5755119	+	618	WP_012477616.1	recombinase family protein	-
LE197_RS27420 (LE197_27415)	5755106..5756242	+	1137	WP_012477617.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	algB	5687935..5905852	217917
	-	flank	IS/Tn	-	-	5753794..5754360	566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10654.14 Da
• Isoelectric Point: 9.6866

>T218661 WP_180690175.1 NZ_CP084484:c5751899-5751618 [Pseudomonas sp. PS1(2021)]
MRTIEQTSRFKRDYKREAKGPHRQTLASDFIAVVTALANNQPLAEKHRDHALAGDWKDHRDCHIKPDLVLIYRKADDAVL
QLVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T218661 NZ_CP116071:c3758812-3758510 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9448.91 Da
• Isoelectric Point: 4.6017

>AT218661 WP_180690176.1 NZ_CP084484:c5752149-5751886 [Pseudomonas sp. PS1(2021)]
MSANAVVRARIDENIKEEATVVLAAMGLTVSDAFRIMLTRVAREKALPFEPLIPNATTIEAMKEARRGGLKSFASVEDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT218661 NZ_CP116071:c3758474-3758133 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG