Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 3625946..3626532 Replicon chromosome
Accession NZ_CP084484
Organism Pseudomonas sp. PS1(2021)

Toxin (Protein)


Gene name PumA Uniprot ID G8CP73
Locus tag LE197_RS17390 Protein ID WP_003120987.1
Coordinates 3626233..3626532 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID -
Locus tag LE197_RS17385 Protein ID WP_003448662.1
Coordinates 3625946..3626236 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LE197_RS17370 (LE197_17370) 3622219..3624195 + 1977 WP_016851611.1 DEAD/DEAH box helicase -
LE197_RS36905 3624205..3624339 + 135 WP_255253641.1 hypothetical protein -
LE197_RS17375 (LE197_17375) 3624336..3624680 + 345 WP_226034088.1 hypothetical protein -
LE197_RS17380 (LE197_17380) 3624720..3625700 + 981 WP_019437692.1 IS5-like element ISPpu21 family transposase -
LE197_RS17385 (LE197_17385) 3625946..3626236 - 291 WP_003448662.1 putative addiction module antidote protein Antitoxin
LE197_RS17390 (LE197_17390) 3626233..3626532 - 300 WP_003120987.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LE197_RS17395 (LE197_17395) 3626734..3627306 + 573 WP_226034089.1 PilL N-terminal domain-containing protein -
LE197_RS17400 (LE197_17400) 3627523..3627723 + 201 WP_226034109.1 TcpQ domain-containing protein -
LE197_RS17405 3628308..3628691 + 384 Protein_3467 secretin N-terminal domain-containing protein -
LE197_RS17410 3628804..3629562 + 759 WP_226034090.1 PilN family type IVB pilus formation outer membrane protein -
LE197_RS17415 (LE197_17410) 3629566..3630891 + 1326 WP_003099758.1 type 4b pilus protein PilO2 -
LE197_RS17420 (LE197_17415) 3630881..3631414 + 534 WP_003099760.1 type IV pilus biogenesis protein PilP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3594362..3703726 109364
- flank IS/Tn - - 3624720..3625700 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-90)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11133.88 Da        Isoelectric Point: 10.4495

>T218659 WP_003120987.1 NZ_CP084484:c3626532-3626233 [Pseudomonas sp. PS1(2021)]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Download         Length: 300 bp

>T218659 NZ_CP116071:2875786-2875893 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10394.91 Da        Isoelectric Point: 4.7527

>AT218659 WP_003448662.1 NZ_CP084484:c3626236-3625946 [Pseudomonas sp. PS1(2021)]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Download         Length: 291 bp

>AT218659 NZ_CP116071:c2875733-2875672 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G8CP73

Antitoxin

Source ID Structure

References