Detailed information of TA system TA218655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1989167..1989976
• Replicon: chromosome
• Accession: NZ_CP084480
• Organism: Staphylococcus lugdunensis strain RMLUG1

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: LFR23_RS09620
• Protein ID: WP_002458758.1
• Coordinates: 1989806..1989976 (+)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: A0A853V5R2
• Locus tag: LFR23_RS09615
• Protein ID: WP_002458757.1
• Coordinates: 1989167..1989766 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFR23_RS09595 (LFR23_09590)	1984754..1986211	+	1458	WP_002477974.1	ABC transporter substrate-binding protein/permease	-
LFR23_RS09600 (LFR23_09595)	1986204..1986929	+	726	WP_002477975.1	amino acid ABC transporter ATP-binding protein	-
LFR23_RS09605 (LFR23_09600)	1987387..1988529	+	1143	WP_094449181.1	tRNA epoxyqueuosine(34) reductase QueG	-
LFR23_RS09610 (LFR23_09605)	1988531..1989001	+	471	WP_002458756.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
LFR23_RS09615 (LFR23_09610)	1989167..1989766	+	600	WP_002458757.1	glucosamine-6-phosphate isomerase	Antitoxin
LFR23_RS09620 (LFR23_09615)	1989806..1989976	+	171	WP_002458758.1	SAS053 family protein	Toxin
LFR23_RS09625 (LFR23_09620)	1990149..1990544	+	396	WP_002458759.1	hypothetical protein	-
LFR23_RS09630 (LFR23_09625)	1990672..1992057	+	1386	WP_002492636.1	class II fumarate hydratase	-
LFR23_RS09635 (LFR23_09630)	1992122..1992958	-	837	WP_002477978.1	RluA family pseudouridine synthase	-
LFR23_RS09640 (LFR23_09635)	1993126..1994238	+	1113	WP_002492606.1	GAF domain-containing sensor histidine kinase	-
LFR23_RS09645 (LFR23_09640)	1994260..1994883	+	624	WP_002492614.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (131-195)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6565.10 Da
• Isoelectric Point: 4.6723

>T218655 WP_002458758.1 NZ_CP084480:1989806-1989976 [Staphylococcus lugdunensis]
MSKEKKSRLNYHEEENAMVENLEDLKELGKEMEHISEKNDEEKVNQSHDTSKSSNS

Nucleotide

• Length: 171 bp

>T218655 NZ_CP116071:2244334-2244436 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22619.51 Da
• Isoelectric Point: 4.9539

>AT218655 WP_002458757.1 NZ_CP084480:1989167-1989766 [Staphylococcus lugdunensis]
MAMNFKVFENKDQVAEYSADIIRKQFNNNPTTIAGFHLNKEASPVLDMLKKNVDQHAVDFSQINILDYDQNQEYFKALGV
PESQIYPISYDQDAEAFISDKIKTKDNKGKLILQVASIDDQGNLNISLRQGLLNAREIILVITGHNKKEVVEKLYNENGK
SNFEPADLKAHRMVTVILDEAAAEGLPEDVRHYFTDRFA

Nucleotide

• Length: 600 bp

>AT218655 NZ_CP116071:c2244474-2244206 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A853V5R2