Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1835429..1835946 Replicon chromosome
Accession NZ_CP084480
Organism Staphylococcus lugdunensis strain RMLUG1

Toxin (Protein)


Gene name mazF Uniprot ID A0A853V3R6
Locus tag LFR23_RS08720 Protein ID WP_002461192.1
Coordinates 1835596..1835946 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A853V233
Locus tag LFR23_RS08715 Protein ID WP_002461193.1
Coordinates 1835429..1835599 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFR23_RS08690 (LFR23_08685) 1831254..1831736 + 483 WP_002492078.1 PH domain-containing protein -
LFR23_RS08695 (LFR23_08690) 1831729..1833243 + 1515 WP_002492082.1 PH domain-containing protein -
LFR23_RS08700 (LFR23_08695) 1833230..1833742 + 513 WP_002461197.1 PH domain-containing protein -
LFR23_RS08705 (LFR23_08700) 1833754..1834146 + 393 WP_002461196.1 holo-ACP synthase -
LFR23_RS08710 (LFR23_08705) 1834194..1835342 + 1149 WP_002492086.1 alanine racemase -
LFR23_RS08715 (LFR23_08710) 1835429..1835599 + 171 WP_002461193.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LFR23_RS08720 (LFR23_08715) 1835596..1835946 + 351 WP_002461192.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LFR23_RS08725 (LFR23_08720) 1836379..1837380 + 1002 WP_002461191.1 PP2C family protein-serine/threonine phosphatase -
LFR23_RS08730 (LFR23_08725) 1837471..1837797 + 327 WP_002461190.1 anti-sigma factor antagonist -
LFR23_RS08735 (LFR23_08730) 1837799..1838278 + 480 WP_037540680.1 anti-sigma B factor RsbW -
LFR23_RS08740 (LFR23_08735) 1838253..1839023 + 771 WP_002461188.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13079.20 Da        Isoelectric Point: 9.9784

>T218653 WP_002461192.1 NZ_CP084480:1835596-1835946 [Staphylococcus lugdunensis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIGKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSEDKMKEVDHALDISLGLHNYH

Download         Length: 351 bp

>T218653 NZ_CP116071:c2043851-2043564 [Escherichia coli]
ATGTTACCCATTTTGTGGCTACCATCTGCTCGCGATGATTTGCGTCAGATCGTAGCCTATATTGCTAAGGAAAATATTCC
TGCAGCACGCAGACTAAAAATACGGATTGAAACGTCTGTTTTAGCCCTCTCTGAGCATCCATATCTATATCCGCCAAGTG
ATCGAGTATCCGGTTTGCGGGAAATTGTGGTTCACCCTAATTATATCGTTTTGTACCGAGTAGCAACTTCAAGCATTGAA
ATTGCAAATATTGTGCATGCCCGCCGACAATTTCCCTTCCCTATCTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6327.92 Da        Isoelectric Point: 4.0115

>AT218653 WP_002461193.1 NZ_CP084480:1835429-1835599 [Staphylococcus lugdunensis]
MLTFNQNRSHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNETYLTSNHQSE

Download         Length: 171 bp

>AT218653 NZ_CP116071:c2044042-2043851 [Escherichia coli]
ATGAGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTACGCGC
TAAAGTGGCGTCAAGCCTTGCAGACCCTCGTCCCTCAATTCCACATGATGAGGTAATGGCTGAAATGGAGAATCTTATTG
CTCAAATTGCTGTAACTAACAGGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853V3R6

Antitoxin

Source ID Structure
AlphaFold DB A0A853V233

References