Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1824031..1824549 Replicon chromosome
Accession NZ_CP084480
Organism Staphylococcus lugdunensis strain RMLUG1

Toxin (Protein)


Gene name TsdT Uniprot ID A0A853V532
Locus tag LFR23_RS08660 Protein ID WP_002492077.1
Coordinates 1824337..1824549 (+) Length 71 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A853V130
Locus tag LFR23_RS08655 Protein ID WP_002492098.1
Coordinates 1824031..1824324 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFR23_RS08635 (LFR23_08630) 1819631..1820269 + 639 WP_002492090.1 thiamine phosphate synthase -
LFR23_RS08640 (LFR23_08635) 1820359..1821234 + 876 WP_002461212.1 membrane protein insertase YidC -
LFR23_RS08645 (LFR23_08640) 1821618..1822259 - 642 WP_002492092.1 HD domain-containing protein -
LFR23_RS08650 (LFR23_08645) 1822381..1823865 - 1485 WP_002478972.1 cardiolipin synthase -
LFR23_RS08655 (LFR23_08650) 1824031..1824324 + 294 WP_002492098.1 copper-sensing transcriptional repressor CsoR Antitoxin
LFR23_RS08660 (LFR23_08655) 1824337..1824549 + 213 WP_002492077.1 heavy metal-associated domain-containing protein Toxin
LFR23_RS08665 (LFR23_08660) 1824640..1824777 + 138 WP_002461206.1 Lmo0850 family protein -
LFR23_RS08670 (LFR23_08665) 1825191..1826381 - 1191 WP_002461205.1 FtsW/RodA/SpoVE family cell cycle protein -
LFR23_RS08675 (LFR23_08670) 1826737..1827807 + 1071 WP_002461204.1 D-alanine--D-alanine ligase -
LFR23_RS08680 (LFR23_08675) 1827825..1829183 + 1359 WP_002492089.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(19-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-90)


Sequences

Toxin        


Download         Length: 71 a.a.        Molecular weight: 8087.02 Da        Isoelectric Point: 3.9947

>T218652 WP_002492077.1 NZ_CP084480:1824337-1824549 [Staphylococcus lugdunensis]
VEKRLIYITNVTNEDDLENLRTRLLEMIGVNEVVINFDERAVNVTFETSTNLNALEKEIYDAGYHAIDDL

Download         Length: 213 bp

>T218652 NZ_CP116071:c1887370-1886996 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11177.85 Da        Isoelectric Point: 6.6367

>AT218652 WP_002492098.1 NZ_CP084480:1824031-1824324 [Staphylococcus lugdunensis]
MNEQDHAHHSKIVKANLKSRLNRIEGQVRAINRMVEDDVYCDDVLTQIRATRSALTSVATKLLEHHMKSCIMDKINDGQE
VEAMEELLVTFQKMLKD

Download         Length: 294 bp

>AT218652 NZ_CP116071:c1887827-1887459 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853V532

Antitoxin

Source ID Structure
AlphaFold DB A0A853V130

References