Detailed information of TA system TA218651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1989068..1989877
• Replicon: chromosome
• Accession: NZ_CP084444
• Organism: Staphylococcus lugdunensis strain RMLUG6

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: LE189_RS09615
• Protein ID: WP_002458758.1
• Coordinates: 1989707..1989877 (+)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: A0A853V5R2
• Locus tag: LE189_RS09610
• Protein ID: WP_002458757.1
• Coordinates: 1989068..1989667 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LE189_RS09590 (LE189_09585)	1984655..1986112	+	1458	WP_002477974.1	ABC transporter substrate-binding protein/permease	-
LE189_RS09595 (LE189_09590)	1986105..1986830	+	726	WP_002477975.1	amino acid ABC transporter ATP-binding protein	-
LE189_RS09600 (LE189_09595)	1987288..1988430	+	1143	WP_094449181.1	tRNA epoxyqueuosine(34) reductase QueG	-
LE189_RS09605 (LE189_09600)	1988432..1988902	+	471	WP_002458756.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
LE189_RS09610 (LE189_09605)	1989068..1989667	+	600	WP_002458757.1	glucosamine-6-phosphate isomerase	Antitoxin
LE189_RS09615 (LE189_09610)	1989707..1989877	+	171	WP_002458758.1	SAS053 family protein	Toxin
LE189_RS09620 (LE189_09615)	1990050..1990445	+	396	WP_002458759.1	hypothetical protein	-
LE189_RS09625 (LE189_09620)	1990573..1991958	+	1386	WP_002492636.1	class II fumarate hydratase	-
LE189_RS09630 (LE189_09625)	1992023..1992859	-	837	WP_002477978.1	RluA family pseudouridine synthase	-
LE189_RS09635 (LE189_09630)	1993027..1994139	+	1113	WP_002492606.1	GAF domain-containing sensor histidine kinase	-
LE189_RS09640 (LE189_09635)	1994161..1994784	+	624	WP_002492614.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (131-195)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6565.10 Da
• Isoelectric Point: 4.6723

>T218651 WP_002458758.1 NZ_CP084444:1989707-1989877 [Staphylococcus lugdunensis]
MSKEKKSRLNYHEEENAMVENLEDLKELGKEMEHISEKNDEEKVNQSHDTSKSSNS

Nucleotide

• Length: 171 bp

>T218651 NZ_CP116071:1876923-1877177 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22619.51 Da
• Isoelectric Point: 4.9539

>AT218651 WP_002458757.1 NZ_CP084444:1989068-1989667 [Staphylococcus lugdunensis]
MAMNFKVFENKDQVAEYSADIIRKQFNNNPTTIAGFHLNKEASPVLDMLKKNVDQHAVDFSQINILDYDQNQEYFKALGV
PESQIYPISYDQDAEAFISDKIKTKDNKGKLILQVASIDDQGNLNISLRQGLLNAREIILVITGHNKKEVVEKLYNENGK
SNFEPADLKAHRMVTVILDEAAAEGLPEDVRHYFTDRFA

Nucleotide

• Length: 600 bp

>AT218651 NZ_CP116071:1876675-1876926 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A853V5R2