Detailed information of TA system TA218650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1886884..1887419
• Replicon: chromosome
• Accession: NZ_CP084444
• Organism: Staphylococcus lugdunensis strain RMLUG6

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: C5QLS6
• Locus tag: LE189_RS08970
• Protein ID: WP_002445368.1
• Coordinates: 1887096..1887419 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: C5QLS5
• Locus tag: LE189_RS08965
• Protein ID: WP_001058489.1
• Coordinates: 1886884..1887093 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LE189_RS08930 (LE189_08925)	1882140..1882424	+	285	WP_002461410.1	co-chaperone GroES	-
LE189_RS08935 (LE189_08930)	1882484..1884106	+	1623	WP_002461409.1	chaperonin GroEL	-
LE189_RS08940 (LE189_08935)	1884175..1885347	-	1173	WP_002492309.1	tyrosine-type recombinase/integrase	-
LE189_RS08945 (LE189_08940)	1885361..1886035	-	675	WP_002445345.1	helix-turn-helix transcriptional regulator	-
LE189_RS08950 (LE189_08945)	1886208..1886426	+	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
LE189_RS08955 (LE189_08950)	1886431..1886748	+	318	WP_002445364.1	helix-turn-helix domain-containing protein	-
LE189_RS08960 (LE189_08955)	1886745..1886891	+	147	WP_002445365.1	hypothetical protein	-
LE189_RS08965 (LE189_08960)	1886884..1887093	+	210	WP_001058489.1	hypothetical protein	Antitoxin
LE189_RS08970 (LE189_08965)	1887096..1887419	+	324	WP_002445368.1	DUF1474 family protein	Toxin
LE189_RS08975 (LE189_08970)	1887483..1888352	+	870	WP_002445370.1	primase alpha helix C-terminal domain-containing protein	-
LE189_RS08980 (LE189_08975)	1888366..1890075	+	1710	WP_002445371.1	DUF927 domain-containing protein	-
LE189_RS08985 (LE189_08980)	1890423..1890788	+	366	WP_002445372.1	hypothetical protein	-
LE189_RS08990 (LE189_08985)	1890802..1891443	+	642	WP_002492310.1	pathogenicity island protein	-
LE189_RS08995 (LE189_08990)	1891731..1892075	+	345	WP_094449183.1	pathogenicity island protein	-
LE189_RS09000 (LE189_08995)	1892089..1892322	+	234	WP_002492317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	1882140..1900417	18277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 13107.51 Da
• Isoelectric Point: 4.8571

>T218650 WP_002445368.1 NZ_CP084444:1887096-1887419 [Staphylococcus lugdunensis]
MNWEIKNLFSDLKLLKDSFEDLKDNHGWHFEELYPHEPNHNLNKDELIREGFSYHERRIHNNQMFDLFHLYIEQFDKIIQ
KFHELEKASSENFGEESDDAQKLKITE

Nucleotide

• Length: 324 bp

>T218650 NZ_CP116071:1876675-1876926 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8225.24 Da
• Isoelectric Point: 4.2820

>AT218650 WP_001058489.1 NZ_CP084444:1886884-1887093 [Staphylococcus lugdunensis]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVDGIEVVSEVSREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT218650 NZ_CP116071:1876923-1877177 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A853V3H1

Antitoxin

• AlphaFold DB: A0A853V601