Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1835330..1835847 Replicon chromosome
Accession NZ_CP084444
Organism Staphylococcus lugdunensis strain RMLUG6

Toxin (Protein)


Gene name mazF Uniprot ID A0A853V3R6
Locus tag LE189_RS08715 Protein ID WP_002461192.1
Coordinates 1835497..1835847 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A853V233
Locus tag LE189_RS08710 Protein ID WP_002461193.1
Coordinates 1835330..1835500 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LE189_RS08685 (LE189_08680) 1831155..1831637 + 483 WP_002492078.1 PH domain-containing protein -
LE189_RS08690 (LE189_08685) 1831630..1833144 + 1515 WP_002492082.1 PH domain-containing protein -
LE189_RS08695 (LE189_08690) 1833131..1833643 + 513 WP_002461197.1 PH domain-containing protein -
LE189_RS08700 (LE189_08695) 1833655..1834047 + 393 WP_002461196.1 holo-ACP synthase -
LE189_RS08705 (LE189_08700) 1834095..1835243 + 1149 WP_002492086.1 alanine racemase -
LE189_RS08710 (LE189_08705) 1835330..1835500 + 171 WP_002461193.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LE189_RS08715 (LE189_08710) 1835497..1835847 + 351 WP_002461192.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LE189_RS08720 (LE189_08715) 1836280..1837281 + 1002 WP_002461191.1 PP2C family protein-serine/threonine phosphatase -
LE189_RS08725 (LE189_08720) 1837372..1837698 + 327 WP_002461190.1 anti-sigma factor antagonist -
LE189_RS08730 (LE189_08725) 1837700..1838179 + 480 WP_037540680.1 anti-sigma B factor RsbW -
LE189_RS08735 (LE189_08730) 1838154..1838924 + 771 WP_002461188.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13079.20 Da        Isoelectric Point: 9.9784

>T218649 WP_002461192.1 NZ_CP084444:1835497-1835847 [Staphylococcus lugdunensis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIGKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSEDKMKEVDHALDISLGLHNYH

Download         Length: 351 bp

>T218649 NZ_CP116071:1039706-1040041 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6327.92 Da        Isoelectric Point: 4.0115

>AT218649 WP_002461193.1 NZ_CP084444:1835330-1835500 [Staphylococcus lugdunensis]
MLTFNQNRSHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNETYLTSNHQSE

Download         Length: 171 bp

>AT218649 NZ_CP116071:1039458-1039706 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853V3R6

Antitoxin

Source ID Structure
AlphaFold DB A0A853V233

References