Detailed information of TA system TA218643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2496908..2497426
• Replicon: chromosome
• Accession: NZ_CP084440
• Organism: Staphylococcus lugdunensis strain RMLUG4

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: LE164_RS11800
• Protein ID: WP_002478973.1
• Coordinates: 2496908..2497120 (-)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A853V130
• Locus tag: LE164_RS11805
• Protein ID: WP_002492098.1
• Coordinates: 2497133..2497426 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LE164_RS11780 (LE164_11795)	2492274..2493632	-	1359	WP_002478974.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
LE164_RS11785 (LE164_11800)	2493650..2494720	-	1071	WP_267262610.1	D-alanine--D-alanine ligase	-
LE164_RS11790 (LE164_11805)	2495076..2496266	+	1191	WP_002461205.1	FtsW/RodA/SpoVE family cell cycle protein	-
LE164_RS11795 (LE164_11810)	2496680..2496817	-	138	WP_002461206.1	Lmo0850 family protein	-
LE164_RS11800 (LE164_11815)	2496908..2497120	-	213	WP_002478973.1	heavy metal-associated domain-containing protein	Toxin
LE164_RS11805 (LE164_11820)	2497133..2497426	-	294	WP_002492098.1	copper-sensing transcriptional repressor CsoR	Antitoxin
LE164_RS11810 (LE164_11825)	2497592..2499076	+	1485	WP_002478972.1	cardiolipin synthase	-
LE164_RS11815 (LE164_11830)	2499198..2499839	+	642	WP_002478971.1	HD domain-containing protein	-
LE164_RS11820 (LE164_11835)	2500223..2501098	-	876	WP_002461212.1	membrane protein insertase YidC	-
LE164_RS11825 (LE164_11840)	2501188..2501826	-	639	WP_002478970.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (19-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-90)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8070.03 Da
• Isoelectric Point: 3.9947

>T218643 WP_002478973.1 NZ_CP084440:c2497120-2496908 [Staphylococcus lugdunensis]
VEKRLIYITNVTSEDDLENLRTRLLEMIGVNEVVINFDERAVNVTFETPTNLNALEKEIYDAGYHAIDDL

Nucleotide

• Length: 213 bp

>T218643 NZ_CP116070:12310-12588 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACCGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11177.85 Da
• Isoelectric Point: 6.6367

>AT218643 WP_002492098.1 NZ_CP084440:c2497426-2497133 [Staphylococcus lugdunensis]
MNEQDHAHHSKIVKANLKSRLNRIEGQVRAINRMVEDDVYCDDVLTQIRATRSALTSVATKLLEHHMKSCIMDKINDGQE
VEAMEELLVTFQKMLKD

Nucleotide

• Length: 294 bp

>AT218643 NZ_CP116070:12041-12310 [Escherichia coli]
ATGACACAGGTTAACATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTTCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A853V130