Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2485508..2486025 Replicon chromosome
Accession NZ_CP084440
Organism Staphylococcus lugdunensis strain RMLUG4

Toxin (Protein)


Gene name mazF Uniprot ID A0A853V3R6
Locus tag LE164_RS11740 Protein ID WP_002461192.1
Coordinates 2485508..2485858 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A853V233
Locus tag LE164_RS11745 Protein ID WP_002461193.1
Coordinates 2485855..2486025 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LE164_RS11720 (LE164_11735) 2482430..2483200 - 771 WP_002461188.1 RNA polymerase sigma factor SigB -
LE164_RS11725 (LE164_11740) 2483175..2483654 - 480 WP_002478979.1 anti-sigma B factor RsbW -
LE164_RS11730 (LE164_11745) 2483656..2483982 - 327 WP_002461190.1 anti-sigma factor antagonist -
LE164_RS11735 (LE164_11750) 2484073..2485074 - 1002 WP_002461191.1 PP2C family protein-serine/threonine phosphatase -
LE164_RS11740 (LE164_11755) 2485508..2485858 - 351 WP_002461192.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LE164_RS11745 (LE164_11760) 2485855..2486025 - 171 WP_002461193.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LE164_RS11750 (LE164_11765) 2486112..2487260 - 1149 WP_012990786.1 alanine racemase -
LE164_RS11755 (LE164_11770) 2487308..2487700 - 393 WP_002461196.1 holo-ACP synthase -
LE164_RS11760 (LE164_11775) 2487712..2488224 - 513 WP_002478976.1 PH domain-containing protein -
LE164_RS11765 (LE164_11780) 2488211..2489725 - 1515 WP_002461198.1 PH domain-containing protein -
LE164_RS11770 (LE164_11785) 2489718..2490200 - 483 WP_002478975.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13079.20 Da        Isoelectric Point: 9.9784

>T218642 WP_002461192.1 NZ_CP084440:c2485858-2485508 [Staphylococcus lugdunensis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIGKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSEDKMKEVDHALDISLGLHNYH

Download         Length: 351 bp

>T218642 NZ_CP116070:2572-2859 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACATAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6327.92 Da        Isoelectric Point: 4.0115

>AT218642 WP_002461193.1 NZ_CP084440:c2486025-2485855 [Staphylococcus lugdunensis]
MLTFNQNRSHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNETYLTSNHQSE

Download         Length: 171 bp

>AT218642 NZ_CP116070:2324-2575 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853V3R6

Antitoxin

Source ID Structure
AlphaFold DB A0A853V233

References