Detailed information of TA system TA218640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 605478..606287
• Replicon: chromosome
• Accession: NZ_CP084437
• Organism: Staphylococcus lugdunensis strain RMLUG3

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: LE166_RS03125
• Protein ID: WP_002458758.1
• Coordinates: 606117..606287 (+)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: A0A853V5R2
• Locus tag: LE166_RS03120
• Protein ID: WP_002458757.1
• Coordinates: 605478..606077 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LE166_RS03100 (LE166_03100)	601065..602522	+	1458	WP_002477974.1	ABC transporter substrate-binding protein/permease	-
LE166_RS03105 (LE166_03105)	602515..603240	+	726	WP_002477975.1	amino acid ABC transporter ATP-binding protein	-
LE166_RS03110 (LE166_03110)	603698..604840	+	1143	WP_094449181.1	tRNA epoxyqueuosine(34) reductase QueG	-
LE166_RS03115 (LE166_03115)	604842..605312	+	471	WP_002458756.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
LE166_RS03120 (LE166_03120)	605478..606077	+	600	WP_002458757.1	glucosamine-6-phosphate isomerase	Antitoxin
LE166_RS03125 (LE166_03125)	606117..606287	+	171	WP_002458758.1	SAS053 family protein	Toxin
LE166_RS03130 (LE166_03130)	606460..606855	+	396	WP_002458759.1	hypothetical protein	-
LE166_RS03135 (LE166_03135)	606983..608368	+	1386	WP_002492636.1	class II fumarate hydratase	-
LE166_RS03140 (LE166_03140)	608433..609269	-	837	WP_002477978.1	RluA family pseudouridine synthase	-
LE166_RS03145 (LE166_03145)	609437..610549	+	1113	WP_002492606.1	GAF domain-containing sensor histidine kinase	-
LE166_RS03150 (LE166_03150)	610571..611194	+	624	WP_002492614.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (131-195)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6565.10 Da
• Isoelectric Point: 4.6723

>T218640 WP_002458758.1 NZ_CP084437:606117-606287 [Staphylococcus lugdunensis]
MSKEKKSRLNYHEEENAMVENLEDLKELGKEMEHISEKNDEEKVNQSHDTSKSSNS

Nucleotide

• Length: 171 bp

>T218640 NZ_CP116068:2555-2830 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACTCGAGAAAACAGGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22619.51 Da
• Isoelectric Point: 4.9539

>AT218640 WP_002458757.1 NZ_CP084437:605478-606077 [Staphylococcus lugdunensis]
MAMNFKVFENKDQVAEYSADIIRKQFNNNPTTIAGFHLNKEASPVLDMLKKNVDQHAVDFSQINILDYDQNQEYFKALGV
PESQIYPISYDQDAEAFISDKIKTKDNKGKLILQVASIDDQGNLNISLRQGLLNAREIILVITGHNKKEVVEKLYNENGK
SNFEPADLKAHRMVTVILDEAAAEGLPEDVRHYFTDRFA

Nucleotide

• Length: 600 bp

>AT218640 NZ_CP116068:2271-2555 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A853V5R2