Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 451642..452159 Replicon chromosome
Accession NZ_CP084437
Organism Staphylococcus lugdunensis strain RMLUG3

Toxin (Protein)


Gene name mazF Uniprot ID A0A853V3R6
Locus tag LE166_RS02225 Protein ID WP_002461192.1
Coordinates 451809..452159 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A853V233
Locus tag LE166_RS02220 Protein ID WP_002461193.1
Coordinates 451642..451812 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LE166_RS02195 (LE166_02195) 447471..447953 + 483 WP_002492078.1 PH domain-containing protein -
LE166_RS02200 (LE166_02200) 447946..449460 + 1515 WP_002492082.1 PH domain-containing protein -
LE166_RS02205 (LE166_02205) 449447..449863 + 417 WP_267262505.1 PH domain-containing protein -
LE166_RS02210 (LE166_02210) 449967..450359 + 393 WP_002461196.1 holo-ACP synthase -
LE166_RS02215 (LE166_02215) 450407..451555 + 1149 WP_002492086.1 alanine racemase -
LE166_RS02220 (LE166_02220) 451642..451812 + 171 WP_002461193.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LE166_RS02225 (LE166_02225) 451809..452159 + 351 WP_002461192.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LE166_RS02230 (LE166_02230) 452593..453594 + 1002 WP_002461191.1 PP2C family protein-serine/threonine phosphatase -
LE166_RS02235 (LE166_02235) 453685..454011 + 327 WP_002461190.1 anti-sigma factor antagonist -
LE166_RS02240 (LE166_02240) 454013..454492 + 480 WP_267262506.1 anti-sigma B factor RsbW -
LE166_RS02245 (LE166_02245) 454467..455237 + 771 WP_002461188.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13079.20 Da        Isoelectric Point: 9.9784

>T218638 WP_002461192.1 NZ_CP084437:451809-452159 [Staphylococcus lugdunensis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIGKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSEDKMKEVDHALDISLGLHNYH

Download         Length: 351 bp

>T218638 NZ_CP116067:4838481-4838783 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6327.92 Da        Isoelectric Point: 4.0115

>AT218638 WP_002461193.1 NZ_CP084437:451642-451812 [Staphylococcus lugdunensis]
MLTFNQNRSHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNETYLTSNHQSE

Download         Length: 171 bp

>AT218638 NZ_CP116067:4838819-4839160 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853V3R6

Antitoxin

Source ID Structure
AlphaFold DB A0A853V233

References